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首页> 外文期刊>Scientific reports. >The Effect of SkitoSnack, an Artificial Blood Meal Replacement, on Aedes aegypti Life History Traits and Gut Microbiota
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The Effect of SkitoSnack, an Artificial Blood Meal Replacement, on Aedes aegypti Life History Traits and Gut Microbiota

机译：SkitoSnack，一种人工代餐食品，对埃及伊蚊生活史特征和肠道菌群的影响

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Public health research and vector control frequently require the rearing of large numbers of vector mosquitoes. All target vector mosquito species are anautogenous, meaning that females require vertebrate blood for egg production. Vertebrate blood, however, is costly, with a short shelf life. To overcome these constraints, we have developed SkitoSnack, an artificial blood meal replacement for the mosquito Aedes aegypti, the vector of dengue, Zika and chikungunya virus. SkitoSnack contains bovine serum albumin and hemoglobin as protein source as well as egg yolk and a bicarbonate buffer. SkitoSnack-raised females had comparable life history traits as blood-raised females. Mosquitoes reared from SkitoSnack-fed females had similar levels of infection and dissemination when orally challenged with dengue virus type 2 (DENV-2) and significantly lower infection with DENV-4. When SkitoSnack was used as a vehicle for DENV-2 delivery, blood-raised and SkitoSnack-raised females were equally susceptible. The midgut microbiota differed significantly between mosquitoes fed on SkitoSnack and mosquitoes fed on blood. By rearing 20 generations of Aedes exclusively on SkitoSnack, we have proven that this artificial diet can replace blood in mosquito mass rearing.
机译：公共卫生研究和病媒控制经常需要饲养大量病媒蚊子。所有目标媒介蚊子都是自体的，这意味着雌性需要脊椎动物的血液才能产卵。然而，脊椎动物的血液价格昂贵，保质期短。为了克服这些限制，我们开发了SkitoSnack，一种人造血粉替代品，用于替代埃及伊蚊，登革热，寨卡病毒和基孔肯雅病毒的媒介。 SkitoSnack包含牛血清白蛋白和血红蛋白作为蛋白质来源，以及蛋黄和碳酸氢盐缓冲液。 SkitoSnack饲养的女性具有与血液饲养的女性相当的生活史特征。从斯基托斯纳克（SkitoSnack）喂养的雌性饲养的蚊子在口服2型登革热病毒（DENV-2）攻击时具有相似的感染和传播水平，而DENV-4的感染率则大大降低。当将SkitoSnack用作DENV-2输送工具时，血统和SkitoSnack举起的雌性同样容易受到感染。用SkitoSnack喂食的蚊子和用血液喂食的蚊子，中肠微生物群有显着差异。通过仅在SkitoSnack上饲养20代伊蚊，我们证明了这种人工饮食可以代替蚊子大量饲养中的血液。

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