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首页> 外文期刊>Scientific reports. >Sheathless CE-MS based metabolic profiling of kidney tissue section samples from a mouse model of Polycystic Kidney Disease
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Sheathless CE-MS based metabolic profiling of kidney tissue section samples from a mouse model of Polycystic Kidney Disease

机译：基于鞘管CE-MS的多囊肾病小鼠模型肾脏组织切片代谢谱分析

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Capillary electrophoresis-mass spectrometry (CE-MS) using a sheathless porous tip interface emerged as an attractive tool in metabolomics thanks to its numerous advantages. One of the main advantages compared to the classical co-axial sheath liquid interface is the increased sensitivity, while maintaining the inherent properties of CE, such as a high separation efficiency and low sample consumption. Specially, the ability to perform nanoliter-based injections from only a few microliters of material in the sample vial makes sheathless CE-MS a well-suited and unique approach for highly sensitive metabolic profiling of limited sample amounts. Therefore, in this work, we demonstrate the utility of sheathless CE-MS for metabolic profiling of biomass-restricted samples, namely for 20?μm-thick tissue sections of kidney from a mouse model of polycystic kidney disease (PKD). The extraction method was designed in such a way to keep a minimum sample-volume in the injection vial, thereby still allowing multiple nanoliter injections for repeatability studies. The developed strategy enabled to differentiate between different stages of PKD and as well changes in a variety of different metabolites could be annotated over experimental groups. These metabolites include carnitine, glutamine, creatine, betaine and creatinine. Overall, this study shows the utility of sheathless CE-MS for biomass-limited metabolomics studies.
机译：使用无鞘多孔尖端接口的毛细管电泳-质谱（CE-MS）由于其众多优势而成为代谢组学中一种有吸引力的工具。与经典同轴鞘液界面相比，主要优点之一是灵敏度提高，同时保持了CE的固有特性，例如高分离效率和低样品消耗。特别是，仅从样品瓶中的几微升材料中进行基于纳升的进样的能力，使无鞘CE-MS成为适合有限样品量的高灵敏度代谢谱分析的非常独特的方法。因此，在这项工作中，我们证明了无鞘CE-MS在生物量受限样品的代谢谱分析中的实用性，即从多囊性肾病（PKD）小鼠模型的20？μm厚的肾脏组织切片。提取方法的设计方式应使进样瓶中的样品量保持最小，从而仍允许多次纳升进样以进行重复性研究。所开发的策略能够区分PKD的不同阶段，并且可以在实验组中注释各种不同代谢产物的变化。这些代谢物包括肉碱，谷氨酰胺，肌酸，甜菜碱和肌酸酐。总的来说，这项研究表明无鞘CE-MS在生物量有限的代谢组学研究中的实用性。

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