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首页> 外文期刊>Journal of genetics >Association of AFLP and SSR markers with agronomic and fibre quality traits in Gossypium hirsutum L.
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Association of AFLP and SSR markers with agronomic and fibre quality traits in Gossypium hirsutum L.

机译：AFLP和SSR标记与Gossymium Hirsutum L中的农艺和纤维品质性状的关联。

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Molecular markers linked to QTL contributing to agronomic and fibre quality traits would be useful for cotton improvement. We have attempted to tag yield and fibre quality traits with AFLP and SSR markers using F2 and F3 populations of a cross between two Gossypium hirsutum varieties, PS56-4 and RS2013. Out of 50 AFLP primer combinations and 177 SSR primer pairs tested, 32 AFLP and four SSR primers were chosen for genotyping F2 individuals.Marker-trait associations were studied for eight agronomic and five fibre quality traits through simple and multiple regression analysis (MRA) using a set of 92 AFLP polymorphic loci and four SSR markers. Simple linear regression analysis (SLRA) identified 23 markers for eight different traits whereas multiple regression analysis identified 30 markers for at least one of the 13 traits. SSR marker BNL 3502 was consistently identified to be associated with fibre strength. While all the markers identified in SLRA were also detected in MRA, as many as 16 of the 30 markers were identified to be associated with respective traits in both F2 and F3 generations. The markers explained up to 41 per cent of phenotypic variation for individual traits. A number of markers were found to be associated with multiple traits suggesting clustering of QTLs for fibre quality traits in cotton.
机译：与QTL相关的分子标记有助于农艺和纤维品质性状的分子标记对于棉花改善是有用的。我们尝试使用AFLP和SSR标记的销量和纤维质量特性使用两种Gossypium Hirsutum品种，PS56-4和RS2013之间的交叉的F2和F3群体。在50个AFLP引物组合中和177个SSR引物对中测试，选择32个AFLP和四个SSR引物，用于基因分型F2个体。通过简单和多元回归分析（MRA）使用八个农艺和五种纤维品质性质进行了八个农艺和五种纤维品质。一组92 AFLP多晶型基因座和四个SSR标记。简单的线性回归分析（SLRA）鉴定了八种不同特征的23个标记，而多元回归分析鉴定了至少一个13个特征中的至少一个标记。 SSR标记BNL 3502一致地识别与纤维强度相关。虽然在MRA中也检测到SCRA中鉴定的所有标记，但是鉴定了30种标记中的多达16种与F2和F3代中的各种性状相关。标记对单个特征的表型变异的标记产生高达41％。发现许多标记与多种性状相关，这表明棉花纤维质量特征的QTL的聚类。

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