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首页> 外文期刊>Journal of Medical Microbiology: An Official Journal of the Pathological Society of Great Britain and Ireland >Genetic characterization of Shigella spp. isolated from diarrhoeal and asymptomatic children
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Genetic characterization of Shigella spp. isolated from diarrhoeal and asymptomatic children

机译：Shigella SPP的遗传表征。与腹泻和无症状的孩子分离

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Phenotypic and genetic characteristics of Shigella spp. isolated from diarrhoeal and asymptomatic children aged up to 5 years were analysed in this study. In total, 91 and 17 isolates were identified from diarrhoeal (case) and asymptomatic (control) children, respectively. All the isolates were tested for antimicrobial resistance, the presence of integrons, plasmid-mediated quinolone resistance (PMQR), virulence-associated genes and Shigella pathogenicity island (SH-PAI). The majority of the Shigella spp. from cases (68.1 %) and controls (82.3 %) were found to be resistant to fluoroquinolones. Integron carriage was detected more in cases (76.9 %) than in controls (35.5 %). Atypical class 1 integron was detected exclusively in Shigella flexneri from cases but not from the controls. PMQR genes such as aac(6′)-Ib-cr and qnrS1 were detected in 82.4 and 14.3 % of the isolates from cases and in 53 and 17.6 % in controls, respectively. Shigella isolates from cases as well as from controls were positive for the invasive plasmid antigen H-encoding gene ipaH. The other virulence genes such as virF, sat, setA, setB, sen and ial were detected in Shigella isolates in 80.2, 49.4, 27.4, 27.4, 80.2 and 79.1 % of cases and in 64.7, 52.9, 17.6, 17.6, 64.7 and 64.7 % of controls, respectively. The entire SH-PAI was detected in S. flexneri serotype 2a from cases and controls. In an isolate from a control child, the SH-PAI was truncated. Integrons, PMQR and virulence-encoding genes were detected more frequently in cases than in controls. In diarrhoea endemic areas, asymptomatic carriers may play a crucial role in the transmission of multidrug-resistant Shigella spp. with all the putative virulence genes.
机译：Shigella spp的表型和遗传特征。在本研究中分析了从腹泻和无症状的腹泻和无症状的儿童进行分析。总共91和17个分离物分别从腹泻（病例）和无症状（对照）儿童中鉴定出来。测试所有分离株的抗微生物抗性，整合子的存在，质粒介导的喹啉酮抗性（PMQR），毒力相关基因和志gla致病性岛（SH-PAI）。大多数志贺氏菌SPP。发现来自病例（68.1％）和对照（82.3％）被发现对氟喹诺酮类药物抵抗力。在案例（76.9％）中检测到综合载体比对照（35.5％）。非典型1级整合在志贺氏素斯蒂（Shigella Flexeri）从案例中检测到，但不是来自控制。 PMQR基因如AAC（6'） - IB-CR和QNRS1分别在82.4和14.3％的分离物中检测到来自病例的分离物和对照中的53％和17.6％。从病例以及对照中的志贺氏菌分离为侵袭性质粒抗原H-编码基因IPAH的阳性。在80.2,49.4,27.4,27.4,80.2和79.1％的病例中，在志贺氏菌分离株中检测到诸如VIRF，SAT，SETA，SETB，SEN和IAL等毒力基因，案件中的病例和64.7,52.9,17.6,17.6,64.7和64.7百分比对照。从病例和对照中检测到整个SH-PAI在S. Flexeri血清型2a中检测到。在来自控制儿童的隔离物中，SH-PAI被截断。在案例中比在对照中更频繁地检测到整合子，PMQR和毒力编码基因。在腹泻流行区域中，无症状载体可能在多药抗志贺氏菌SPP的传输中发挥至关重要的作用。所有推定的毒力基因。

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