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首页> 外文期刊>Journal of Medical Microbiology: An Official Journal of the Pathological Society of Great Britain and Ireland >A novel Omp25-binding peptide screened by phage display can inhibit Brucella abortus 2308 infection in vitro and in vivo
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A novel Omp25-binding peptide screened by phage display can inhibit Brucella abortus 2308 infection in vitro and in vivo

机译：通过噬菌体展示筛选的新型OMP25结合肽可以抑制体外和体内Brucella Abortus 2308感染

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摘要

Brucellosis is a globally distributed zoonotic disease affecting animals and humans, and current antibiotic and vaccine strategies are not optimal. The surface-exposed protein Omp25 is involved in Brucella virulence and plays an important role in Brucella pathogenesis during infection, suggesting that Omp25 could be a useful target for selecting potential therapeutic molecules to inhibit Brucella pathogenesis. In this study, we identified, we believe for the first time, peptides that bind specifically to the Omp25 protein of pathogens, using a phage panning technique, After four rounds of panning, 42 plaques of eluted phages were subjected to pyrosequencing. Four phage clones that bound better than the other clones were selected following confirmation by ELISA and affinity constant determination. The peptides selected could significantly inhibit Brucella abortus 2308 (S2308) internalization and intracellular growth in RAW264.7 macrophages, and significantly induce secretion of TNF-α and IL-12 in peptide- and S2308-treated cells. Any observed peptide (OP11, OP27, OP35 or OP40) could significantly inhibit S2308 infection in BALB/c mice. Moreover, the peptide OP11 was the best candidate peptide for inhibiting S2308 infection in vitro and in vivo. These results suggest that peptide OP11 has potential for exploitation as a peptide drug in resisting S2308 infection.
机译：布鲁克病是一种全球分布的人畜共患疾病，影响动物和人类，目前的抗生素和疫苗策略不是最佳的。表面暴露的蛋白质OMP25涉及Brucella毒力，在感染期间在Brucella发病作用中起重要作用，表明OMP25可以是选择潜在治疗分子以抑制布鲁氏菌发病机制的有用靶标。在这项研究中，我们鉴定了，我们认为，使用噬菌体淘选技术的噬菌体淘气技术的肽首次结合的肽，经过四轮平移，将42张洗脱噬菌体进行焦点进行。在通过ELISA确认和亲和恒定测定后选择粘合优于其他克隆的四个噬菌体克隆。选择的肽可以显着抑制Raw264.7巨噬细胞中的Brucella Abortius 2308（S2308）内化和细胞内生长，并显着诱导肽和S2308处理细胞中TNF-α和IL-12的分泌。任何观察到的肽（OP11，OP27，OP35或OP40）可以显着抑制BALB / C小鼠中的S2308感染。此外，肽OP11是用于抑制体外和体内S2308感染的最佳候选肽。这些结果表明，肽OP11具有在抵抗S2308感染时作为肽药物的剥削潜力。

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