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首页> 外文期刊>Journal of Medical Microbiology: An Official Journal of the Pathological Society of Great Britain and Ireland >High frequency of cultivable human subgroup F adenoviruses in stool samples from a paediatric population admitted to hospital with acute gastroenteritis
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High frequency of cultivable human subgroup F adenoviruses in stool samples from a paediatric population admitted to hospital with acute gastroenteritis

机译：高频率的培养人亚组F腺病毒来自急性胃肠炎入院的儿科人群的粪便样本

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The family Adenoviridae consists of five genera of which the genus Mastadenovirus includes human viruses classified into 57 serotypes clustered into seven subgroups (A–G). Serotypes 40 and 41 (subgroup F) are specifically associated with childhood gastroenteritis and are the most common cause of acute gastroenteritis in young children after rotaviruses and noroviruses. Standard methods for laboratory diagnosis of adenovirus infection include electron microscopy (EM) and conventional cell culture (CCC), although it is widely considered that adenoviruses 40 and 41 are difficult to cultivate, such that their circulation is most likely underestimated. One hundred and ten faecal specimens from paediatric patients with gastroenteritis were confirmed positive for adenovirus by EM and/or CCC at the Virology Unit of the University Hospital of Parma, Italy, during the period January 2010–December 2012. They were analysed to determine the actual prevalence of adenovirus 40 and 41 in these patients using PCR and restriction endonuclease analysis, and to evaluate their ability to be cultivated in standard cell lines. The results showed a high prevalence of subgroup F (62.7 %), with serotype 41 (89.8 %) predominating over serotype 40 (10.2 %). Surprisingly, among the 75 adenoviruses isolated by CCC, 37 (49 %) belonged to subgroup F, suggesting a higher capacity of adenovirus 40 and 41 to replicate in cell culture than previously thought. PCR and restriction enzyme techniques provide an efficient means of diagnosing enteric adenoviruses correctly, including subgroup F adenovirus strains in young children with gastroenteritis.
机译：家庭腺病毒含量由五属组成，其中Mastadenovirus属包括分为57个血清型的人类病毒，该组聚集成七个亚组（A-G）。血清型40和41（亚组F）与儿童胃肠炎有关，并且是罗出血血病和诺维病毒后幼儿急性胃肠炎最常见的原因。腺病毒感染的实验室诊断的标准方法包括电子显微镜（EM）和常规细胞培养（CCC），尽管众所周知，腺病毒40和41难以培养，使得它们的循环最可能被低估。来自意大利帕尔马大学医院病毒学单位的EM和/或CCC在2010年1月至2012年12月期间，EM和/或CCC确认了来自胃肠炎的儿科胃肠炎患者的患者对腺病毒的阳性。他们分析了它们以确定使用PCR和限制性内切核酸酶分析的这些患者腺病毒40和41的实际患病率，并评估其在标准细胞系中培养的能力。结果表明，亚组F（62.7％）的普及率高，血清型41（89.8％）占血清型40（10.2％）。令人惊讶的是，在CCC分离的75个腺病毒中，37（49％）属于亚组F，表明腺病毒40和41的较高容量，以在细胞培养中复制而不是先前思考。 PCR和限制酶技术提供了诊断肠道腺病毒的有效方法，包括胃肠炎的幼儿中的亚组腺病毒菌株。

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