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首页> 外文期刊>Journal of Medical Microbiology: An Official Journal of the Pathological Society of Great Britain and Ireland >Comparative analysis of 16S RNA nucleotide sequences of Anaplasma phagocytophilum detected in the blood of horses from various parts of Europe
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Comparative analysis of 16S RNA nucleotide sequences of Anaplasma phagocytophilum detected in the blood of horses from various parts of Europe

机译：欧洲各地血液中检测到的16S RNA核苷酸序列的16S RNA核苷酸序列

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The aim of this study was to conduct a comparative analysis of the 16S rRNA gene fragment nucleotide sequences for Anaplasma phagocytophilum strains detected in the blood of horses from various parts of Europe. The study comprised 234 horses that had had contact with ticks. Using PCR, the genetic material of A. phagocytophilum was identified in the blood of 42 animals. The sequences of the 16S RNA gene amplicons that were obtained from our A. phagocytophilum isolates had 100 % similarity with each other and 96.4–100 % similarity with Anaplasma spp. sequences selected from those available in GenBank. Nucleotide substitutions at positions 248 and 249 were demonstrated in all the 16S RNA gene sequences of Anaplasma obtained in our study. This may indicate the emergence of a new rickettsial genotype that is the cause of equine granulocytic anaplasmosis in southern and eastern Europe. These results add new information on the epidemiology and genetic diversity of A. phagocytophilum detected in the blood of horses from southern and eastern Europe.
机译：该研究的目的是对来自欧洲各地的马匹中检测到的血液中的16S rRNA基因片段核苷酸序列进行比较分析。该研究包括234匹马，与蜱虫接触。使用PCR，在42只动物的血液中鉴定了A.吞噬蛋白的遗传物质。从我们的A.获得的16S RNA基因扩增子的序列具有100％的相似性与Anplasma SPP相似于彼此100％相似之处和96.4-100％相似之处。从Genbank中选择的序列。在我们研究中获得的所有16S RNA基因序列的所有16S RNA基因序列中证明了位置248和249的核苷酸取代。这可能表明，新的Rickettial基因型的出现是南欧南部和东欧的马粒细胞族蛋白病的原因。这些结果为来自南部和东欧血液中检测到的吞噬噬菌体的流行病学和遗传多样性的新信息。

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