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首页> 外文期刊>Journal of Medical Microbiology: An Official Journal of the Pathological Society of Great Britain and Ireland >A mutant in the Listeria monocytogenes Fur-regulated virulence locus (frvA) induces cellular immunity and confers protection against listeriosis in mice
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A mutant in the Listeria monocytogenes Fur-regulated virulence locus (frvA) induces cellular immunity and confers protection against listeriosis in mice

机译：histeria单核细胞增生的突变体毛刺毒力基因座（FRVA）诱导细胞免疫，并赋予小鼠中缺失的保护

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Listeria monocytogenes is a Gram-positive intracellular pathogen that is responsible for listeriosis, a potentially fatal, food-borne illness. Due to its cytoplasmic location during infection, this pathogen can mediate a long-lasting cellular immune response, which makes attenuated strains strong candidates for vaccine development. Recently, our group identified and characterized frvA (Fur-regulated virulence factor A), and deletion of this gene resulted in disruption of iron homeostasis and a strong attenuation in virulence. Despite significant attenuation in the mouse infection model, the frvA mutant was capable of intracellular growth in antigen-presenting cells. Indeed, mice immunized with L. monocytogenes ΔfrvA were able to effectively stimulate specific CD8+ T cells to the listerial epitopes LLO91–99 and P60217–225 at levels comparable with L. monocytogenes strain EGDe. Most notably, mice immunized with ΔfrvA then subsequently challenged with the wild-type strain were completely protected from listerial infection. On the basis of these results, we advocate the use of ΔfrvA as a live attenuated listerial vaccine, and propose that this mutant may serve as a platform for the development of a future vaccine delivery vehicle.
机译：Histeria单核细胞增生是一种革兰氏阳性的细胞内病原体，其负责休闲症，潜在致命，食物疾病。由于其在感染期间的细胞质位置，该病原体可以介导长期的细胞免疫反应，这使得衰减菌株的疫苗发育的强烈候选。最近，我们的组鉴定并表征了FRVA（毛皮调节的毒力因子A），并且缺失该基因导致铁稳态的破坏以及毒力的强衰减。尽管在小鼠感染模型中显着衰减，但FRVA突变体能够在抗原呈递细胞中细胞内生长。实际上，用L.单核细胞增生ΔFRVA免疫的小鼠能够在与L.单核细胞增生株Egde相当的水平上有效地刺激特异性CD8 + T细胞。最值得注意的是，用ΔFrVa免疫的小鼠随后用野生型菌株攻击遭受脑卒中感染的攻击。在这些结果的基础上，我们倡导使用ΔFRVA作为现场衰减的历史疫苗，并提出这种突变体可以作为开发未来疫苗输送车辆的平台。

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