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首页> 外文期刊>Journal of Clinical Microbiology >Identification of major slowly growing pathogenic mycobacteria and Mycobacterium gordonae by high-performance liquid chromatography of their mycolic acids.
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Identification of major slowly growing pathogenic mycobacteria and Mycobacterium gordonae by high-performance liquid chromatography of their mycolic acids.

机译:其氰酸高效液相色谱法鉴定主要慢慢生长致病性分枝杆菌和戈奈戈曲霉。

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摘要

A rapid, reverse-phase high-performance liquid chromatography method was used to detect rho-bromophenacyl mycolic acid ester patterns for strains of four major pathogenic Mycobacterium species and for the most commonly encountered saprophytic species, Mycobacterium gordonae. Mycobacteria in low numbers (2.5 X 10(6) CFU) were detected and identified to the species level. Standard chromatographic patterns characteristic of each species were established. Simple pattern recognition enabled rapid identification of M. tuberculosis, M. kansasii, M. avium, M. intracellulare, and M. gordonae.
机译:快速,反相高效液相色谱法检测四种主要致病性分枝杆菌物种的菌株的Rho-溴苯硫基氨基磺酸酯模式,以及最常见的嗜孢子物种,戈杜杆菌。检测到低数量的分枝杆菌(2.5×10(6)个CFU)并鉴定为物种水平。建立了每种物种的标准色谱图案。简单的图案识别使M. Tuberculosis,M. kansasii,M.Avium,M.细胞内和M. Gordonae的快速鉴定。

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