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首页> 外文期刊>Journal of Clinical Microbiology >Heterogeneity in immunoblot patterns obtained by using four strains of Borrelia burgdorferi and sera from naturally exposed dogs.
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Heterogeneity in immunoblot patterns obtained by using four strains of Borrelia burgdorferi and sera from naturally exposed dogs.

机译：通过使用来自天然暴露的犬的四种苯甲丝伯格多利和血清获得的免疫印迹模式中的异质性。

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摘要

This study evaluated the differences in immunoblot patterns when serum specimens from seropositive dogs were reacted against four strains of Borrelia burgdorferi. Intense bands were consistently detected for the 83-, 66-, 61- to 60-, 41-, and 31-kilodalton regions for all four strains. Most variations were observed in the regions of 45 to 34 and 26 to 15 kilodaltons. Adsorption studies suggested that one reason for the observed variability was a difference in proteins among the organisms, rather than a difference in migration of proteins. Therefore, knowledge and consistency of the test antigen are essential when evaluating and comparing canine immunoblot patterns to B. burgdorferi, but for diagnostic purposes all of the serum samples would have been considered positive regardless of the strain used.
机译：该研究评估了当来自血清阳性犬的血清标本与四种Borrelia Burgdorferi反应时的免疫印迹模式的差异。对于所有四种菌株的83-，66-，61至60-，41-千杆和31千杆杆菌区一直检测到强烈的带。在45至34和26至15千千宫的区域中观察到大多数变化。吸附研究表明，观察到的变异性的一个原因是生物体中蛋白质的差异，而不是蛋白质迁移的差异。因此，在评估和比较犬免疫印迹模式至B.Burgdorferi时，测试抗原的知识和一致性是必要的，但对于诊断目的，所有血清样品都被认为是阳性的，而不管使用的菌株。

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