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Sensitivity of Agar Overlay Method for the Recognition of Enteroviruses

机译:琼脂覆盖方法对肠病患者的敏感性

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Comparison of the agar overlay technique with the fluid culture technique for isolation and identification of enteroviruses showed the former to be useful: (i) for isolation of enterovirus when the number of virus particles was too small to produce detectable cytopathic effect (CPE) in fluid cultures, (ii) for isolation of echovirus 22 which did not produce detectable CPE in fluid cultures, (iii) as an aid to rapid differentiation of enteroviruses, and (iv) for differentiation of viruses in mixed infections. Nonpolio enterovirus isolation experience in the New Haven area over a 4-year period is presented. It was concluded that the agar overlay technique is both useful and relatively simple for routine examination of clinical specimens in a diagnostic laboratory.
机译:琼脂覆盖技术与流体培养技术的分离和鉴定肠病病毒的比较显示,前者是有用的:(i)当病毒颗粒的数量太小而不能在流体中产生可检测的细胞病变效果(CPE)时肠道病毒培养物,(ii)用于分离出在流体培养物中没有产生可检测的CPE,(iii)作为肠病病毒的快速分化,(iv)在混合感染中的病毒分化的辅助性。在4年期间,新避风港肠道肠道病毒隔离体验的非政治性肠道病毒的孤立经验。得出结论是,琼脂覆盖技术在诊断实验室中的临床标本常规检查均具有很有用且相对简单。

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