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首页> 外文期刊>Infection and immunity >Pathogenicity of Theileria parva is influenced by the host cell type infected by the parasite.
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Pathogenicity of Theileria parva is influenced by the host cell type infected by the parasite.

机译：Theileria Parva的致病性受寄生虫感染的宿主细胞类型的影响。

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Theileria parva has been shown to infect and transform B cells and T cells at similar frequencies in vitro. However, the majority of parasitized cells in the tissues of infected cattle are alpha/beta T cells. The aim of this study was to determine whether the cell type infected with T. parva influenced the pathogenicity of the parasite. The initial approach, which involved inoculation of cattle with autologous cloned cell lines of different phenotypes, failed to resolve the issue, because of prolonged period of culture required to clone and characterize the cell lines resulted in attenuation of the cells. As an alternative approach, cattle were inoculated with purified populations of autologous cells that had been incubated in vitro with T. parva sporozoites for 48 h. As few as 3 x 10(4) peripheral blood mononuclear cells (PBMC) treated in this way were found to produce severe clinical reactions with high levels of parasitosis. Infections of similar severity were produced with purified populations of CD2+, CD4+, and CD8+ T cells. By contrast, infected B cells gave rise to mild self-limiting infections even when administered at a 10-fold-higher dose. In animals that received infected CD4+ or CD8+ T cells, the parasitized cells in the lymph nodes on day 11 of infection were all within the CD4+ and CD8+ populations, respectively, indicating that there had been minimal transfer of the parasite between cell types. Phenotypic analyses of cultures of PBMC infected in vitro with saturating concentrations of sporozoites revealed that parasitized B cells were abundant in the cultures after 1 week but were subsequently overgrown by T cells. The results of these experiments indicate that the cell type infected by T. parva influences the pathogenicity of the parasite.
机译：已经显示出在体外类似频率的感染和转化B细胞和T细胞的杂志。然而，受感染牛组织中的大多数寄生细胞是α/βT细胞。本研究的目的是确定感染的细胞类型是否受到T.Parva的影响影响了寄生虫的致病性。涉及与自体克隆的细胞系不同的不同表型的牛接种牛的初始方法未能解决问题，因为克隆所需的培养物长期，并且表征细胞系导致细胞的衰减。作为一种替代方法，用纯化的自体细胞的纯化群接种，其在体外与T.Parva孢子孵育48小时。发现以这种方式处理的少至3×10（4）个外周血单核细胞（PBMC），以产生高水平的寄生虫病毒治疗严重的临床反应。用CD2 +，CD4 +和CD8 + T细胞的纯化群产生类似严重程度的感染。相比之下，即使在10倍的剂量施用时，感染的B细胞也产生了轻度自限制感染。在接受受感染的CD4 +或CD8 + T细胞的动物中，感染第11天的淋巴结中的寄生细胞分别在CD4 +和CD8 +群体中，表明寄生虫在细胞类型之间的转移最小。饱和孢子饱和浓度的体外PBMC培养的表型分析显示，寄生B细胞在1周后培养物中丰富，但随后由T细胞覆盖。这些实验的结果表明，由T.Parva感染的细胞类型会影响寄生虫的致病性。

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