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首页> 外文期刊>Infection and immunity >Cellular immune responses to recombinant heat shock protein 70 from Histoplasma capsulatum.
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Cellular immune responses to recombinant heat shock protein 70 from Histoplasma capsulatum.

机译：细胞免疫应答从组织荚膜囊肿重组热休克蛋白70。

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摘要

Heat shock protein (hsp) 70 from several microbes is antigenic in mammals. In this study we sequenced and expressed the gene encoding this protein from Histoplasma capsulatum to study its immunological activity. The deduced amino acid sequence of the gene demonstrated 71 and 76% identity to hsp7O from humans and Saccharomyces cerevisiae, respectively. A cDNA was synthesized by reverse transcription-PCR and was expressed in Escherichia coli. Recombinant protein reacted with a mouse monoclonal antibody raised against human hsp7O. Splenocytes from C57BL/6 mice immunized with recombinant hsp7O emulsified in adjuvant, but not yeast cells, reacted in vitro to the antigen. Recombinant hsp7O elicited a cutaneous delayed-type hypersensitivity response in mice immunized with protein or with viable yeast cells. Mice were injected with recombinant hsp7O and challenged intranasally with a sublethal inoculum of yeast cells. Vaccination did not confer protection in this model. Thus, recombinant hsp7O can induce a cell-mediated immune response but does not induce a protective response.
机译：来自几个微生物的热休克蛋白（HSP）70在哺乳动物中是抗原性。在该研究中，我们测序并表达了从组织荚膜组织中编码该蛋白质的基因，以研究其免疫活性。该基因的推导的氨基酸序列分别从人和酿酒酵母的Hsp7o证明了71％和76％的同一性。通过逆转录PCR合成cDNA，并在大肠杆菌中表达。重组蛋白与针对人Hsp7O饲养的小鼠单克隆抗体反应。用在佐剂中乳化的重组Hsp7o免疫的C57BL / 6小鼠的脾细胞，但不是酵母细胞，在体外反应到抗原。重组Hsp7O引发了用蛋白质免疫或具有活酵母细胞免疫的小鼠中的皮肤延迟型超敏反应。用重组Hsp7O注射小鼠并用含有酵母细胞的亚麻状细胞鼻内置于鼻内攻击。疫苗接种在该模型中没有授予保护。因此，重组Hsp7O可以诱导细胞介导的免疫应答，但不会引起保护反应。

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