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Porphyromonas gingivalis genes isolated by screening for epithelial cell attachment.

机译:通过筛选上皮细胞附着分离的卟啉核糖属植物基因。

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Porphyromonas gingivalis is associated with chronic and severe periodontitis in adults. P. gingivalis and the other periodontal pathogens colonize and interact with gingival epithelial cells, but the genes and molecular mechanisms involved are unknown. To dissect the first steps in these interactions, a P. gingivalis expression library was screened for clones which bound human oral epithelial cells. Insert DNA from the recombinant clones did not contain homology to the P. gingivalis fimA gene, encoding fimbrillin, the subunit protein of fimbriae, but showed various degrees of homology to certain cysteine protease-hemagglutinin genes. The DNA sequence of one insert revealed three putative open reading frames which appeared to be in an operon. The relationship between P. gingivalis attachment to epithelial cells and the activities identified by the screen is discussed.
机译:Porphyromonas Gingivalis与成年人中的慢性和严重的牙周炎有关。 P. Gingivalis和其他牙周病原体殖民和与牙龈上皮细胞相互作用,但所涉及的基因和分子机制是未知的。解剖这些相互作用中的第一步,筛选了结合人口腔上皮细胞的克隆的P.Gingivalis表达文库。从重组克隆的插入DNA不含对P.Gingivalis FIMA基因的同源性,编码FIMBRILLIN,FIMBRIAE的亚基蛋白,但显示出某些半胱氨酸蛋白酶 - 血凝素基因的各种同源性。一个插入件的DNA序列显示出三个推定的开放阅读框架,它似乎在操纵子中。讨论了P.Gingivalis对上皮细胞附着的关系和屏幕鉴定的活动。

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