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首页> 外文期刊>Infection and immunity >Reactivity of mouse T-cell hybridomas expressing human Vbeta gene segments with staphylococcal and streptococcal superantigens.
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Reactivity of mouse T-cell hybridomas expressing human Vbeta gene segments with staphylococcal and streptococcal superantigens.

机译：用葡萄球菌和链球菌超抗原表达人VBETA基因区段的小鼠T细胞杂交瘤的反应性。

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摘要

A panel of 15 mouse T-cell hybridomas, each expressing a different human Vbeta gene segment (hVbeta) in an otherwise mouse T-cell receptor (i.e., mouse alpha chain and CD3 complex), was constructed by transfection of hVbeta/mouse Cbeta chimeric T-cell receptor (TCR)-beta genes into a mouse T-cell hybridoma recipient lacking the endogenous TCR-beta chain. Several qualities that are conferred by the hVbeta chain of the TCR are retained in the chimeric human-mouse TCR complex: a large panel of hVbeta-specific antibodies specifically stained the hVbeta expressed by the mouse T-cell hybridomas. Moreover, hVbeta-transfected mouse cells could readily produce interleukin 2 when stimulated by superantigens presented by antigen-presenting cells. These characteristics made it possible to refine the reactivity of 17 superantigen preparations with the available transfected Vbetas. Each superantigen gave a characteristic pattern of reactivity on the transfectants. Positive reactivities with some of these transfectants, which differ only by the expressed hVbeta, demonstrate unambiguously the superantigenic character of a protein or fraction and its potential to react with the corresponding Vbetas. Therefore, these hVbeta-transfected cells constituted a valuable tool for determining "specificity fingerprints" of known or putative superantigens. First, commonly used, commercially available superantigens such as staphylococcal enterotoxin B and toxic shock syndrome toxin-1 (TSST-1) showed additional Vbeta reactivities, compared with those of their recombinant counterparts. This stresses the importance of using defined preparations of superantigens for the definition of Vbeta specificities. Second, the stimulatory pattern of a strain of Streptococcus pyogenes demonstrated that this strain, unlike others, produces a potent Vbeta 8-specific superantigen that is an yet undefined at the molecular level.
机译：通过转染HVBETA /小鼠CBETA嵌合体构建了15只小鼠T细胞受体（即小鼠α链和CD3复合物）中表达不同的人VBETA基因区段（HVBeta）的小鼠T细胞杂交瘤。通过转染HVBETA /小鼠CBETA嵌合体T细胞受体（TCR）-beta基因进入小鼠T细胞杂交瘤受体缺乏内源性TCR-β链。 TCR的HVBeta链赋予的几种质量在嵌合人小鼠TCR复合物中保留：大面板的HVBeta特异性抗体特异性染色了小鼠T细胞杂交瘤表达的HVBeta。此外，当通过抗原呈递细胞呈现的超抗原刺激时，HVBeta转染的小鼠细胞可以容易地产生白细胞介素2。这些特性使得可以通过可用的转染的Vbetas优化17种超抗原制剂的反应性。每个超抗原在转染剂上给出了反应性的特征模式。具有这些转染剂中的一些仅由表达的HVBeta不同的阳性反应性，证明了蛋白质或馏分的超占年性特征及其与相应的VBetas反应的可能性。因此，这些转染的电池构成了用于确定已知或推定的超抗原的“特异性指纹”的有价值的工具。首先，常用的商业上可获得的超剧性如葡萄球菌肠毒素B和有毒休克综合征毒素-1（TSST-1）显示出额外的VBeta重塑，与其重组对应物相比。这强调了使用超沉体的定义制剂用于定义VBETA特异性的重要性。其次，链球菌菌株的刺激模式证明这种菌株与其他物体不同，产生有效的VBeta 8特异性超粒子，其在分子水平下尚未确定。

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