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首页> 外文期刊>Infection and immunity >Epitopes recognized by human T lymphocytes in the ROP2 protein antigen of Toxoplasma gondii.
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Epitopes recognized by human T lymphocytes in the ROP2 protein antigen of Toxoplasma gondii.

机译：在弓形虫的ROP2蛋白抗原中的人T淋巴细胞识别的表位。

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摘要

The ROP2 protein of Toxoplasma gondii possesses immunological and biological properties which have led to its proposal as a vaccine candidate. To identify epitopes recognized by human T cells in the ROP2 antigen, we submitted the sequence of this protein to three reported T-cell epitope prediction algorithms. Three sequences that were predicted by all three methods were selected (sequences 197 to 216, 393 to 410, and 501 to 524), and the corresponding peptides were synthesized. The peptides were first tested in a proliferation assay with a DPw4-restricted, ROP2-specific human T-cell clone, and the peptide corresponding to residues 197 to 216 was shown to stimulate the T-cell clone. The three peptides were further tested in proliferation assays with peripheral blood mononuclear cells from a panel of T. gondii-seropositive and -seronegative individuals. We found that cells from a high proportion of the seropositive donors (64%) recognized at least one of the three peptides. The most frequently recognized ones were peptides 197 to 216 (45%) and 501 to 524 (36%). None of the seronegative donors responded to any peptide. These results show that the ROP2 antigen of T. gondii contains T-cell epitopes recognized by a high percentage of the immune population and further strengthen its potential as a vaccine candidate.
机译：弓形虫的ROP2蛋白具有免疫学和生物学性质，其导致其作为疫苗候选的提案。为了鉴定ROP2抗原中人T细胞识别的表位，我们将该蛋白质的序列提交给三个报告的T细胞表位预测算法。选择了所有三种方法预测的三个序列（序列197至216,393-410和501至524），并合成了相应的肽。首先用DPW4限制的ROP2特异性人T细胞克隆在增殖测定中测试肽，并且显示与残基197至216的肽刺激T细胞克隆。在具有来自T.Gondii-Seropolyive和-Seronegative个体面板的外周血单核细胞的增殖测定中进一步测试了三种肽。我们发现来自高比例的血清阳性供体（64％）的细胞识别出三种肽中的至少一种。最常见的识别的肽是197至216（45％）和501-524（36％）。没有一个血管药捐赠者对任何肽作出反应。这些结果表明，T.Gondii的ROP2抗原含有由高百分比的免疫群体认识到的T细胞表位，并进一步加强其作为疫苗候选的潜力。

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