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首页> 外文期刊>Molecular and Cellular Biology >Pam17 Is Required for Architecture and Translocation Activity of the Mitochondrial Protein Import Motor
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Pam17 Is Required for Architecture and Translocation Activity of the Mitochondrial Protein Import Motor

机译：PAM17是线粒体蛋白质进口电机的建筑和易位活动所必需的

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摘要

Import of mitochondrial matrix proteins involves the general translocase of the outer membrane and the presequence translocase of the inner membrane. The presequence translocase-associated motor (PAM) drives the completion of preprotein translocation into the matrix. Five subunits of PAM are known: the preprotein-binding matrix heat shock protein 70 (mtHsp70), the nucleotide exchange factor Mge1, Tim44 that directs mtHsp70 to the inner membrane, and the membrane-bound complex of Pam16-Pam18 that regulates the ATPase activity of mtHsp70. We have identified a sixth motor subunit. Pam17 (encoded by the open reading frame YKR065c) is anchored in the inner membrane and exposed to the matrix. Mitochondria lacking Pam17 are selectively impaired in the import of matrix proteins and the generation of an import-driving activity of PAM. Pam17 is required for formation of a stable complex between the cochaperones Pam16 and Pam18 and promotes the association of Pam16-Pam18 with the presequence translocase. Our findings suggest that Pam17 is required for the correct organization of the Pam16-Pam18 complex and thus contributes to regulation of mtHsp70 activity at the inner membrane translocation site.
机译：线粒体基质蛋白的进口涉及外膜的一般译本和内膜的前易译。 PRESEQUENCE易位和关联电机（PAM）驱动前蛋白易位完成到矩阵。众所周知，PAM的五个亚基：预蛋白结合基质热休克蛋白70（MTHSP70），将MTHSP70指向内膜的核苷酸交换因子MGE1，TIM44，以及调节ATP酶活性的PAM16-PAM18的膜结合复合物Mthsp70。我们已经确定了第六次电机亚基。 PAM17（由开放式读取框架YKR065C编码）锚定在内膜中并暴露于基质。缺乏PAM17的线粒体在基质蛋白质的进口和产生PAM的进口驾驶活动中被选择性受损。 PAM17是在Cochaperone PAM16和PAM18之间形成稳定的综合体，并促进PAM16-PAM18与Presequence译本组合。我们的研究结果表明，PAM16-PAM18复合物的正确组织需要PAM17，从而有助于调节内膜易位位点的MTHSP70活性。

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