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首页> 外文期刊>Journal of Clinical Microbiology >Use of a dispersed repetitive DNA element to distinguish clinical isolates of Cryptococcus neoformans.
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Use of a dispersed repetitive DNA element to distinguish clinical isolates of Cryptococcus neoformans.

机译：使用分散的重复DNA元素来区分临床分离株Neoformornans。

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摘要

We isolated a recombinant phage from a Cryptococcus neoformans genomic library that contains a member of a dispersed family of repetitive DNA elements. This clone, CNRE-1, hybridized to at least seven chromosomes in C. neoformans on the basis of pulsed-field gel analysis. Hybridization of CNRE-1 to restriction digests of genomic DNA confirmed that there are multiple copies of this element and that restriction fragment length polymorphisms are present in strains from different serotypes of C. neoformans. The utility of this probe as an epidemiologic marker was determined by testing cryptococcal isolates from a single hospital. Five isolates from four patients were closely related to a serotype A reference strain, whereas five other isolates from four additional patients exhibited distinct patterns. In two patients, the isolates obtained during recurrent cryptococcal infections were identical to the original isolates.
机译：我们从密集的噬菌体的基因组文库中分离重组噬菌体，其含有分散的重复性DNA元素的成员。该克隆，CNRE-1，在脉冲场凝胶分析的基础上，在C.Neoformans中的至少七种染色体杂交。 CNRE-1的杂交至基因组DNA的限制性消化证实，该元素的多个拷贝，并且限制性片段长度多态性存在于来自C，Neoformans的不同血清型的菌株中。该探针作为流行病学标记物的效用是通过从单个医院的碱性分离物测试的。来自四名患者的五个分离株与血清型密切相关，而来自四名额外患者的其他5个分离株表现出明显的图案。在两名患者中，经常性密集的密集剂感染期间获得的分离物与原始分离株相同。

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