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首页> 外文期刊>Journal of bacteriology >Structure of Escherichia coli After Freeze-Etching
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Structure of Escherichia coli After Freeze-Etching

机译：冷冻蚀刻后大肠杆菌的结构

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摘要

Survival of Escherichia coli, quick-frozen under conditions similar to those employed for freeze-etching, is close to 100%. For determination of cell shrinkage, the diameters of freeze-etched E. coli cells (average, 0.99 μm) were compared with those of preparations after negative staining and after ultrathin sectioning. Negatively stained cells measured from 0.65 to 1.0 μm in diameter, and ultrathin sections showed average cell diameters of 0.70 μm. Freeze-etched replicas of logarithmically growing, as well as stationary, E. coli B cells revealed a smooth, finely pitted cell surface in contrast to cell surfaces seen with other preparative methods. The frozen cell wall may cleave in two planes, exposing (i) a smooth fracture face within the lipid layer and (ii) in rare instances an ill-defined particulate layer. Most frequently, however, cleavage of the envelope occurred between wall and protoplasmic membrane; large areas of the membrane were then exposed and showed a surface studded with predominantly spherical particles, an appearance which did not significantly change when the cells were fixed in formaldehyde and osmium tetroxide before freeze-etching. The distribution of these particles differed between logarithmically growing cells and stationary cells.
机译：大肠杆菌的存活率，在类似于用于冻蚀的条件下快速冷冻，接近100％。用于测定细胞收缩，冻结蚀刻的直径 E。将Coli 细胞（平均，0.99μm）与负染色后的制剂和超薄切片后进行比较。呈染色的细胞直径为0.65至1.0μm，超薄部分显示平均细胞直径为0.70μm。冻结对数生长的复制品，以及静止， E。 COLI B细胞显示出与用其他制备方法所见的细胞表面相比光滑的细胞表面。冷冻细胞壁可以在两个平面中切割，暴露（i）在罕见的颗粒层中的脂质层和（ii）内的平滑骨折面。然而，最常见的是，在壁和原生质膜之间发生封装的裂解;然后暴露出膜的大面积，并显示出镶嵌具有主要球形颗粒的表面，当细胞在甲醛和十六烷烃固定在冻蚀刻时没有显着改变的外观。这些颗粒的分布在对数生长的细胞和静止细胞之间不同。

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