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首页> 外文期刊>Journal of bacteriology >Stabilizing Effect of Colicin E2 on the “Spheroplast Membrane”
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Stabilizing Effect of Colicin E2 on the “Spheroplast Membrane”

机译：肠溶蛋白E2对“纺血塑膜”的稳定作用

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Cells of Escherichia coli W3110 and its thymineless mutant, both of which are colicin E2 sensitive, were treated with colicin E2, and then converted to spheroplasts. These spheroplasts seemed to be more stable than those from untreated cells; suspensions of spheroplasts of untreated cells were lysed spontaneously and the turbidity was reduced by approximately 45% on incubation with ethylenediaminetetraacetic acid-lysozyme, whereas suspensions of spheroplasts of colicin E2-treated cells showed 25% reduction in turbidity. This change was irreversible and 5 min of treatment with colicin E2 at 37 C was necessary for stabilization. This process was inhibited by 2,4-dinitrophenol or streptomycin. Cells harboring the colicin E2 factor were not affected by treatment in this way with colicin E2. Alteration of composition of phospholipids was not observed.
机译：大肠杆菌Coli W3110及其胸腺轴突变体的细胞，两者都是敏菌素E2敏感性，用肠E2处理，然后转化为Spurhoplasts。这些螺旋状体似乎比来自未经处理的细胞的球质形成更稳定;未经处理的细胞的悬浮体自发地裂解，与乙二胺四乙酸 - 溶菌酶一起孵育浊度约45％，而粘性蛋白E2处理细胞的悬浮蛋白的悬浮体显示出浊度的25％。这种变化是不可逆转的，并且在37℃下用肠道E2处理5分钟的稳定性。该过程受2,4-二硝基苯酚或链霉素抑制。通过这种方式与肠道E2的治疗不影响含有肠E2因子的细胞。未观察到磷脂组合物的改变。

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