Partial Characterization of Aerolysin, a Lytic Exotoxin from Aeromonas hydrophila

Alan W. Bernheimer; Lois S. Avigad

首页> 外文期刊>Infection and immunity >Partial Characterization of Aerolysin, a Lytic Exotoxin from Aeromonas hydrophila

【24h】

Partial Characterization of Aerolysin, a Lytic Exotoxin from Aeromonas hydrophila

机译：Aeromonas患有Aeromonas疏水素的裂解外毒素的部分表征

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Conditions are defined for the production, in high titers, of an extracellular hemolytic toxin of Aeromonas hydrophila, here termed “aerolysin.” Substantial purification of the toxin was accomplished by means of salt fractionation, dialysis, and gel filtration, with a yield of 24% of the starting activity. Analysis of the product by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate revealed a single, heavy protein band and a number of faint protein bands. The estimated molecular weight of the heavy band (50,000) was in close agreement with that (53,000) of the substance responsible for hemolytic activity as determined by gel filtration. Purified aerolysin is a labile substance, apparently protein. It is not inactivated by any of several proteases under the conditions employed nor is it inhibited by any of several lipids tested. About 0.1 μg administered to mice intravenously is lethal. The physical properties of aerolysin show considerable resemblance to those described for the exotoxin of Pseudomonas aeruginosa.

机译：在这里称为 Aeromonas疏水层的细胞外溶血性毒素的生产，在HINGRALLASS HERTOPIRA 中定义了条件。通过盐分级，透析和凝胶过滤完成毒素的大量纯化，产率为24％的起始活性。通过聚丙烯酰胺凝胶电泳在十二烷基硫酸钠存在下通过聚丙烯酰胺凝胶电泳分析，揭示了单一，重蛋白条带和许多微弱的蛋白质带。估计的重带（50,000）的分子量与由凝胶过滤测定的溶血活性的物质密切一致，如凝胶过滤所确定的那样。纯化的Areatysin是一种不稳定的物质，显然是蛋白质。在所用条件下的任何一种蛋白酶下，它并不是灭活，也不是由几种测试的脂质的任何脂质抑制。静脉内施用于小鼠的约0.1μg是致命的。 Aerolysin的物理性质表明，对假单胞菌铜绿假单胞菌的外毒素描述的那些相当相似。 展开▼

著录项

来源
《Infection and immunity》 |1974年第6期|共6页

作者
Alan W. Bernheimer; Lois S. Avigad;
展开▼

作者单位

展开▼

收录信息

原文格式 PDF

正文语种

中图分类医学免疫学;

关键词

相似文献

外文文献

中文文献

专利

1. Marker exchange mutagenesis of the aerolysin determinant in Aeromonas hydrophila demonstrates the role of aerolysin in A. hydrophila-associated systemic infections. [J] . T Chakraborty, B Huhle, H Hof, Infection and immunity . 1987,第9期

机译：嗜水气单胞菌中气溶素决定簇的标记交换诱变证明了气溶素在嗜水气单胞菌相关的全身性感染中的作用。

2. Gene Cloning, Expression, and Characterization of Recombinant Aerolysin from Aeromonas hydrophila [J] . Vijai Singh, Pallavi Somvanshi, Gaurav Rathore, Applied Biochemistry and Biotechnology . 2010,第7期

机译：嗜水气单胞菌重组气溶素的基因克隆，表达及鉴定

3. Gene Cloning, Expression, and Characterization of Recombinant Aerolysin from Aeromonas hydrophila [J] . Vijai Singh, Pallavi Somvanshi, Gaurav Rathore, Applied biochemistry and biotechnology, Part A. enzyme engineering and biotechnology . 2010,第7期

机译：嗜水气单胞菌重组气溶素的基因克隆，表达及鉴定

4. Increased sensitivity in the detection and quantitation of virulence factors of Aeromonas hydrophila and Aeromonas caviae using quantitative PCR [C] . V. C. Ardi, B. H. Olson International Conference on Environmental Problems in Coastal Regions: Incorporating Oil Spill Studies; 2004; Unversity of Alicante(ES) . 2004

机译：使用定量PCR提高对嗜水气单胞菌和豚鼠气单胞菌毒力因子的检测和定量的敏感性

5. Heme regulation of the cytolytic enterotoxin/aerolysin/hemolysin expression in Aeromonas hydrophila and prevalence of the toxin gene amongst Aeromonas coastal water populations. [D] . Ardi, Veronica Chinta. 2005

机译：血红素调节嗜水气单胞菌中溶细胞性肠毒素/气溶素/溶血素的表达，以及毒素基因在沿海气单胞菌种群中的流行。

6. Partial Characterization of Aerolysin a Lytic Exotoxin from Aeromonas hydrophila [O] . Alan W. Bernheimer, Lois S. Avigad 1974

机译：嗜水气单胞菌的溶菌性外毒素Aerolysin的部分表征

7. Partial Characterization of Aerolysin, a Lytic Exotoxin from Aeromonas hydrophila [O] . Alan W. Bernheimer, Lois S. Avigad 1974

机译：Aeromonas患有Aeromonas疏水素的裂解外毒素的部分表征

8. Aeromonas Hydrophila as an Agent of Infection in Alligators. Phase II, Final Report; Phase III, Partial Report. Progress Report, September 1, 1976--September 30, 1977, Part II [R] . Gorden, R. W., Esch, G. W. 1977

机译：嗜水气单胞菌作为鳄鱼感染的一种药剂。第二阶段，最终报告;第三阶段，部分报告。进展报告，1976年9月1日 - 1977年9月30日，第二部分

1. 虹鳟源嗜水气单胞菌(Aeromonas hydrophila)分离及16SrRNA基因鉴定 [J] . 郭中钢 ,王恒 ,李良玉 . 水产科技情报 . 2020,第001期

2. Aeromonas veronii biovar veronii and sepsis-infrequent complication of biliary drainage placement: A case report [J] . Manlio Monti1 ,Arianna Torri2 ,Elena Amadori3 . 世界临床病例杂志 . 2019,第006期

3. pH和NaCl质量浓度对发酵鱼糜中腐败菌Aeromonas veronii bv.veronii群体感应的影响 [J] . 赵丹丹 ,刘琳 ,王迪 . 食品与发酵工业 . 2018,第002期

4. In Vitro Antibacterial Potential of Plant Extracts and in Vivo Ilex paraguariensis Effect on Oreochromis niloticus Physiology and Resistance to Aeromonas hydrophila [J] . Micheli Becker1 ,Maria Alice Nunes1 ,Vanessa Gomes da Silva1 . 农业科学与技术：B . 2018,第007期

5. 斑点叉尾鮰(Ictalunes punctatus)源中间气单胞菌(Aeromonas media)分离鉴定及药敏特性 [J] . 杨移斌 ,杨秋红 ,胥宁 . 海洋与湖沼 . 2016,第006期

6. 一株溶藻气单胞菌(Aeromonas sp.)的溶藻特性 [C] . 刘玉梅 ,王梦蕙 ,李力 . 第二届IWA-中国水环境嗅味问题及控制技术国际研讨会暨第五届“黄河杯”城镇饮用水安全保障技术交流会 . 2011

7. 灞河气单胞菌(Aeromonas)抗生素耐药性和重金属抗性研究 [A] . 朱泽亮 . 2020

1. Aeromonas aquariorum ZX1在还原硫酸盐中的应用 [P] . 中国专利： CN104512969B . 2016.09.07

2. Aeromonas aquariorum ZX1在还原硫酸盐中的应用 [P] . 中国专利： CN104512969A . 2015-04-15

3. NOVEL AEROMONAS HYDROPHILA BACTERIOPHAGE AER-HYP-3 AND USE THEREOF FOR INHIBITING GROWTH OF AEROMONAS HYDROPHILA BACTERIA [P] . 外国专利： US2021085733A1 . 2021-03-25

机译：新型航空肺肝脏噬菌体AER-HYP-3及其用途，用于抑制Aeromonas疏水菌细菌的生长

4. NOVEL AEROMONAS HYDROPHILA BACTERIOPHAGE AER-HYP-3 AND USE THEREOF FOR INHIBITING GROWTH OF AEROMONAS HYDROPHILA BACTERIA [P] . 外国专利： WO2019164195A1 . 2019-08-29

机译：新型嗜水气单胞菌噬菌体Aer-HYP-3及其用于抑制嗜水气单胞菌的生长

5. NOVEL AEROMONAS HYDROPHILA BACTERIOPHAGE Aer-HYP-3 AND USE THEREOF FOR INHIBITING GROWTH OF AEROMONAS HYDROPHILA BACTERIA [P] . 外国专利： KR102012227B1 . 2019-08-21

机译：新型嗜水气单胞菌噬菌体Aer-HYP-3及其用于抑制嗜水气单胞菌的生长

相关主题

Partial Characterization of Aerolysin, a Lytic Exotoxin from Aeromonas hydrophila

摘要

著录项

相似文献

相关主题

期刊订阅