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Purification and Some Properties of Progenitor Toxins of Clostridium botulinum Type B

机译：菌株肉毒杆菌型祖毒素纯化和一些性质B.

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Purification of progenitor toxin of Clostridium botulinum type B strain Okra was undertaken by sequential steps of acid precipitation, extraction, ammonium sulfate precipitation, ribonuclease digestion, acid precipitation, protamine treatment, sulphopropyl-Sephadex chromatography, and Sephadex G-200 gel filtration. Two different molecular-sized toxins, named large (L) and medium (M) toxins, were obtained. L toxin was centrifugally homogeneous but electrophoretically heterogeneous. It contained 2.5 × 108 to 3.0 × 108 mean lethal doses per mg of nitrogen, and its sedimentation constant was 16S. M toxin was centrifugally and electrophoretically homogeneous. It contained 5.5 × 108 to 6.0 × 108 mean lethal doses per mg of nitrogen, and its sedimentation constant was 12S. The presence of both L and M toxins in spent culture was demonstrated. It seems justified, therefore, to call both progenitor toxins. Both consisted of toxic and nontoxic components. The toxic components of L and M toxins appeared to be identical with each other. The nontoxic component of L toxin was 12S and possessed a hemagglutinin activity of about 0.5% that of type A crystalline toxin; that of M toxin was 7S and possessed no hemagglutinin activity. They were antigenically related but not identical.
机译：通过顺序步骤酸沉淀，萃取，硫酸铵沉淀，核核酸酶消化，酸沉淀，硫酸胍处理，磺基丙酯 - Sephadex色谱，酸沉淀，硫酸钙溶液，磺基丙酯 - Sephadex色谱和Sephadex G和Sephadex G的顺序步骤进行了血管肉蛋白的血管肉蛋白的毒素毒素汤隆型B型菌株OKRA型B型菌株Okra。 -200凝胶过滤。获得了两种不同的分子大小毒素，命名为大（L）和培养基（M）毒素。 l毒素是离心均匀的但电泳异质的。它含有2.5×10 8 至3.0×10 8 / sup>平均致死剂量每mg氮，其沉降常数为16 s 。 M毒素在离心和电泳上均匀。它含有5.5×10 8 至6.0×10 8 平均致死剂量为每mg氮，其沉降常数为12 s 。展示了L和M毒素的存在在花培养中。因此，似乎是祖先毒素的合理性。两者都是由有毒和无毒的组分组成。 L和M毒素的有毒成分似乎彼此相同。 L毒素的无毒成分为12℃，并具有约0.5％的型结晶毒素的血凝素活性; m毒素的那种 s 并没有血凝素活动。它们是抗原相关但不相同的。

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