...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Identification of Rickettsia rickettsii in formalin-fixed, paraffin-embedded tissues by immunofluorescence.
【24h】

Identification of Rickettsia rickettsii in formalin-fixed, paraffin-embedded tissues by immunofluorescence.

机译：免疫荧光鉴定福尔马林固定的石蜡嵌入式组织中的Rickettsia Rickettsii。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

With slight modification of a trypsin digestion technique, Rickettsia rickettsii were demonstrated specifically by immunofluorescence staining in Formalin-fixed, paraffin-embedded tissue sections from a human, rhesus monkey, and guinea pig with Rocky Mountain spotted fever and in infected membranes from a chicken embryo. Tissues were cut at 4 micron and, using geltain as a tissue adhesive, were hydrated in a routine manner. Sections were then digested in refrigerated 0.1% trypsin for 16 h, washed, and stained specifically for R. rickettsii by direct or indirect immunofluorescence. Rickettsial organisms were localized in affected vessels of the mammalian species and within the yolk sac epithelium of the chicken embryo. Specificity was confirmed by adsorbing antibody conjugates with R. rickettsii organisms. Trypsin digestion probably decreased tissue proteins which interfered with immunochemical attachment of antibody to the rickettsiae. The technique is valuable in that a diagnosis of Rocky Mountain spotted fever can be confirmed from Formalin-fixed tissues processed in a routine manner.
机译：随着胰蛋白酶消化技术的轻微修饰，Rickettsia Rickettsii通过来自人，恒河猴和豚鼠的福尔马林固定的，石蜡嵌入的组织切片中的免疫荧光染色，与鸡山斑点发烧和来自鸡胚的感染膜。将组织切割为4微米，并以甘露出剂以常规方式水合。然后在冷藏0.1％胰蛋白酶中消化切片，通过直接或间接免疫荧光进行洗涤，并专门为R.Rickettsii染色。羊皮氏生物在哺乳动物物种的受影响血管中局部化，并在鸡胚的卵黄囊上皮内。通过吸附抗体缀合物与R.Rickettsii生物体的抗体缀合物证实了特异性。胰蛋白酶消化可能减少了组织蛋白质，其干扰了抗体与Rickettsiae的免疫化学附着。该技术具有重要的是，可以从常规方式从福尔马林固定组织中从福尔马林固定组织中确认落基山斑发热的诊断。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号