• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Scientific reports. >Combination of ESI and MALDI mass spectrometry for qualitative, semi-quantitative and in situ analysis of gangliosides in brain
【24h】

Combination of ESI and MALDI mass spectrometry for qualitative, semi-quantitative and in situ analysis of gangliosides in brain

机译：ESI和MALDI质谱法的组合对大脑神经节神经节的定性，半定量和原位分析

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Gangliosides are a family of complex lipids that are abundant in the brain. There is no doubt the investigations about the distribution of gangliosides in brian and the relationship between gangliosides and Alzheimer's disease is profound. However, these investigations are full of challenges due to the structural complexity of gangliosides. In this work, the method for efficient extraction and enrichment of gangliosides from brain was established. Moreover, the distribution of gangliosides in brain was obtained by matrix-assisted laser desorption ionization (MALDI) mass spectrometry imaging (MSI). It was found that 3-aminoquinoline (3-AQ) as matrix was well-suited for MALDI MS analysis of gangliosides in negative ion mode. In addition, the pretreatment by ethanol (EtOH) cleaning brain section and the addition of ammonium formate greatly improved the MS signal of gangliosides in the brain section when MALDI MSI analysis was employed. The distribution of ganliosides in cerebral cortex, hippocampus and cerebellum was respectively acquired by electrospray ionization (ESI) MS and MALDI MSI, and the data were compared for reliability evaluation of MALDI MSI. Further, applying MALDI MSI technology, the distribution of gangliosides in amyloid precursor protein transgenic mouse brain was obtained, which may provide a new insight for bioresearch of Alzheimer's disease (AD).
机译：神经节苷脂是大脑中丰富的复杂脂质家族。毫无疑问，对Brian和神经节苷脂和阿尔茨海默病之间的关系的调查是深刻的。然而，由于神经节苷脂的结构性复杂性，这些调查充满了挑战。在这项工作中，建立了从大脑中有效提取和富集神经节苷脂的方法。此外，通过基质辅助激光解吸电离（MALDI）质谱成像（MSI）获得脑的神经节苷脂的分布。发现3-氨基喹啉（3-AQ）作为基质非常适合于负离子模式下对神经节素的MALDI MS分析。此外，当使用MALDI MSI分析时，乙醇（EtOH）清洁脑截面和添加铵甲酸的添加大大改善了脑部中神经节素的MS信号。通过电喷雾电离（ESI）MS和MALDI MSI，分别获得脑皮质，海马和小脑菌中的GANLIOSIDS的分布，并将数据进行比较，以便对MALDI MSI的可靠性评估进行比较。此外，获得MALDI MSI技术，获得了淀粉样蛋白前体蛋白转基因小鼠脑的神经节苷脂的分布，这可能为阿尔茨海默病（AD）的生物研究提供了新的洞察。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号