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首页> 外文期刊>Scientific reports. >Genomic and metagenomic signatures of giant viruses are ubiquitous in water samples from sewage, inland lake, waste water treatment plant, and municipal water supply in Mumbai, India
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Genomic and metagenomic signatures of giant viruses are ubiquitous in water samples from sewage, inland lake, waste water treatment plant, and municipal water supply in Mumbai, India

机译：巨型病毒的基因组和偏心组织特征在污水，内陆湖，废水处理厂和印度孟买市政供水和市政供水中普遍存在

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We report the detection of genomic signatures of giant viruses (GVs) in the metagenomes of three environment samples from Mumbai, India, namely, a pre-filter of a household water purifier, a sludge sample from wastewater treatment plant (WWTP), and a drying bed sample of the same WWTP. The de novo assembled contigs of each sample yielded 700 to 2000 maximum unique matches with the GV genomic database. In all three samples, the maximum number of reads aligned to Pandoraviridae, followed by Phycodnaviridae, Mimiviridae, Iridoviridae, and other Megaviruses. We also isolated GVs from every environmental sample (n?=?20) we tested using co-culture of the sample with Acanthomoeba castellanii. From this, four randomly selected GVs were subjected to the genomic characterization that showed remarkable cladistic homology with the three GV families viz., Mimivirirdae (Mimivirus Bombay [MVB]), Megaviruses (Powai lake megavirus [PLMV] and Bandra megavius [BAV]), and Marseilleviridae (Kurlavirus [KV]). All 4 isolates exhibited remarkable genomic identity with respective GV families. Functionally, the genomes were indistinguishable from other previously reported GVs, encoding nearly all COGs across extant family members. Further, the uncanny genomic homogeneity exhibited by individual GV families across distant geographies indicate their yet to be ascertained ecological significance.
机译：我们报告检测来自孟买，印度的三种环境样本的巨型病毒（GVS）的基因组特征，即家用净水器的预过滤器，废水处理厂（WWTP）的污泥样品和A干燥床样品相同的扭转液。每个样本的DE Novo组装的Contigs会产生700至2000个与GV基因组数据库的最大独特匹配。在所有三种样品中，与Pandoraviridae对齐的最大读数，其次是Phycodnaviridae，Mimiviridae，Iridoviridae和其他巨脂血清。我们还使用与acanthomoeba castellanii的样品的共同培养进行测试的每个环境样本（n？= 20）孤立的gvs。由此，对四种随机选择的GVS进行基因组表征，其与三种GV家族viz显示出显着的粘层同源性。，Mimivirirdae（Mimivirus Bombay [MVB]），MegaViruses（Powai Lake Megavirus [Plmv]和Bandra Megavius [BAV]） ，和马赛维尼（Kurlavirus [kv]）。所有4个分离物与各自的GV家族表现出显着的基因组特征。在功能上，基因组与其他先前报告的GV来说是难以区分的，几乎所有患有现存家庭成员的齿轮都会编码。此外，各个GV家族跨越地区呈现的不可思议的基因组均匀性表明其尚未确定的生态意义。

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