• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Balkan journal of medical genetics: BJMG >?2-Elemene inhibits the proliferation and migration of human glioblastoma cell lines via suppressing ring finger protein 135
【24h】

?2-Elemene inhibits the proliferation and migration of human glioblastoma cell lines via suppressing ring finger protein 135

机译：？2-榄烯烯通过抑制无胶指蛋白135抑制人胶质母细胞瘤细胞系的增殖和迁移

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

?2-Elemene is commonly used as an anti-cancer agent in different types of cancers and its effects on glioblastoma have been studied through different pathways. However, its effect through ring finger protein 135 (RNF135, OMIM 611358) (RNF135), which is upregulated in glioblastomas, has not yet been explored. The current study is focused on the effects of ?2-elemene on human glioblastoma cell lines U251, U118, A172 and U87 through RNF13 5. A cell counting kit-8 assay and wound healing assay have been utilized to test the proliferation and migration of the cells. Western blot and quantitative real-time-polymerase chain reaction (qRT-PCR) were used to evaluate the level of expression of RNF135. A model of nude mice was used to explore progression of the tumor in vivo. It was observed that increasing treatment time or dose of ?2-elemene remarkably decreased viability of the cells. The cells that were treated with ?2-elemene had a much lower speed of moving toward the gap in comparison to untreated cell lines. ?2-Elemene-treated cells showed a much lower level of expression of RNF135 mRNA than control groups (p 0.05) and the levels of RNF135 protein were lower in the cells treated with ?2-elemene than in control groups (p 0.05). Moreover, tumor progression in subcutaneous xenograft nude mice was delayed with the injection of ?2-elemene. Altogether, our findings suggest that ?2-elemene inhibits proliferation, migration and tumorigenicity of human glioblastoma cells through suppressing RNF135.
机译：？2-榄烯烯通常用作不同类型的癌症的抗癌剂，并通过不同的途径研究了对胶质母细胞瘤的影响。然而，其通过无胶印蛋白质135（RNF135，OMIM 611358）（RNF135）的效果尚未探讨在GlioBlastomas中上调。目前的研究专注于α2-榄烯烯对人胶质母细胞系细胞系U251，U118，A172和U87至RNF13的影响。用于测试增殖和迁移的细胞计数试剂盒-8测定和伤口愈合测定。细胞。用于评估RNF135的表达水平的Western印迹和定量的实时聚合酶链反应（QRT-PCR）。使用裸鼠模型用于探讨体内肿瘤的进展。观察到，增加治疗时间或剂量的α2-榄烯的可吸收性显着降低。用β2-榄烯处理的细胞具有与未处理的细胞系相比朝向间隙移动的速度较低。 ？2- eLemene处理的细胞显示RNF135 mRNA的表达水平大于对照组（P <0.05），并且在用β-榄烯烯处理的细胞中较低的RNF135蛋白水平而不是对照组（P <0.05 ）。此外，皮下异种移植裸鼠的肿瘤进展随注射β2-榄烯烯。我们的研究结果完全表明，通过抑制RNF135，2-eLemeNe抑制人胶质母细胞瘤细胞的增殖，迁移和致瘤性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号