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首页> 外文期刊>BMC Ophthalmology >Blue-light filtering alters angiogenic signaling in human retinal pigmented epithelial cells culture model
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Blue-light filtering alters angiogenic signaling in human retinal pigmented epithelial cells culture model

机译：蓝光过滤改变人视网膜色素上皮细胞培养模型中的血管生成信号

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Light exposure and more specifically the spectrum of blue light contribute to the oxidative stress in Age-related macular degeneration (AMD). The purpose of the study was to establish whether blue light filtering could modify proangiogenic signaling produced by retinal pigmented epithelial (RPE) cells under different conditions simulating risk factors for AMD. Three experiments were carried out in order to expose ARPE-19 cells to white light for 48?h with and without blue light-blocking filters (BLF) in different conditions. In each experiment one group was exposed to light with no BLF protection, a second group was exposed to light with BLF protection, and a control group was not exposed to light. The ARPE-19 cells used in each experiment prior to light exposure were cultured for 24?h as follows: Experiment 1) Normoxia, Experiment 2) Hypoxia, and Experiment 3) Lutein supplemented media in normoxia. The media of all groups was harvested after light exposure for sandwich ELISA-based assays to quantify 10 pro-angiogenic cytokines. A significant decrease in angiogenin secretion levels and a significant increase in bFGF were observed following light exposure, compared to dark conditions, in both normoxia and hypoxia conditions. With the addition of a blue light-blocking filter in normoxia, a significant increase in angiogenin levels was observed. Although statistical significance was not achieved, blue light filters reduce light-induced secretion of bFGF and VEGF to near normal levels. This trend is also observed when ARPE-19 cells are grown under hypoxic conditions and when pre-treated with lutein prior to exposure to experimental conditions. Following light exposure, there is a decrease in angiogenin secretion by ARPE-19 cells, which was abrogated with a blue light - blocking filter. Our findings support the position that blue light filtering affects the secretion of angiogenic factors by retinal pigmented epithelial cells under normoxic, hypoxic, and lutein-pretreated conditions in a similar manner.
机译：曝光和更具体地，蓝光的光谱有助于年龄相关性黄斑变性（AMD）的氧化应激。该研究的目的是建立蓝光过滤是否可以在模拟AMD的危险因素的不同条件下通过视网膜着色上皮（RPE）细胞产生的过致原性信号传导。进行三个实验，以使ARPE-19细胞暴露于白光48Ω，在不同条件下没有蓝色遮光滤光器（BLF）。在每个实验中，一组暴露于没有BLF保护的光线，用BLF保护暴露第二组，并且对照组没有暴露于光线。在曝光前的每种实验中使用的ARPE-19细胞如下培养：实验1）常氧，实验2）缺氧，实验3）促丁蛋白补充媒介常氧介质。在光线暴露的基于ELISA的测定后收获所有组的介质，以定量10个促血管生成细胞因子。在常氧和缺氧条件相比，在轻曝光和缺氧条件下，观察到血管生成素分泌水平和BFGF显着增加的显着降低。随着常氧中的蓝色遮阻过滤器添加，观察到血管生成素水平的显着增加。尽管未实现统计显着性，但蓝光滤光器将光诱导的BFGF和VEGF分泌降低到正常水平附近。当ARPE-19细胞在缺氧条件下并且在暴露于实验条件之前用叶黄素预处理时，也观察到这种趋势。在曝光之后，ARPE-19细胞的血管生成素分泌有降低，其用蓝色遮光滤光器消除。我们的研究结果支持蓝色光学滤波通过在常氧，缺氧和叶黄素预处理条件下通过视网膜色素上皮细胞影响血管生成因子的分泌。

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