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首页> 外文期刊>BMC Infectious Diseases >Emergence of norovirus GII.P16-GII.2 strains in patients with acute gastroenteritis in Huzhou, China, 2016–2017
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Emergence of norovirus GII.P16-GII.2 strains in patients with acute gastroenteritis in Huzhou, China, 2016–2017

机译：诺洛病毒GII.P16-GII.2在中国湖州，中国湖州急性胃肠炎患者中出现菌株，2016 - 2017年

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In late 2016, an uncommon recombinant NoV genotype called GII.P16-GII.2 caused a sharp increase in outbreaks of acute gastroenteritis in different countries of Asia and Europe, including China. However, we did not observe a drastic increase in sporadic norovirus cases in the winter of 2016 in Huzhou. Therefore, we investigate the prevalence and genetic diversity of NoVs in the sporadic acute gastroenteritis (AGE) cases from January 2016 to December 2017 in Huzhou City, Zhejiang, China. From January 2016 to December 2017, a total of 1001 specimens collected from patients with AGE were screened for NoV by real-time RT-PCR. Partial sequences of the RNA-dependent RNA polymerase (RdRp) and capsid gene of the positive samples were amplified by RT-PCR and sequenced. Genotypes of NoV were confirmed by online NoV typing tool and phylogenetic analysis. Complete VP1 sequences of GII.P16-GII.2 strains detected in this study were further obtained and subjected into sequence analysis. In total, 204 (20.4%) specimens were identified as NoV-positive. GII genogroup accounted for most of the NoV-infected cases (98.0%, 200/204). NoV infection was found in all age groups tested (60?years), with the 5-15?year age group having the highest detection rate (17/49, 34.7%). Higher activity of NoV infection could be seen in winter-spring season. The predominant NoV genotypes have changed from GII.Pe-GII.4 Sydney2012 and GII.P17-GII.17 in 2016 to GII.P16-GII.2, GII.Pe-GII.4 Sydney2012 and GII.P17-GII.17 in 2017. Phylogenetic analyses revealed that 2016-2017 GII.P16-GII.2 strains were most closely related to Japan 2010-2012 cluster in VP1 region and no common mutations were found in the amino acids of the HBGA-binding sites and the predicted epitopes. We report the emergence of GII.P16-GII.2 strains and characterize the molecular epidemiological patterns NoV infection between January 2016 and December 2017 in Huzhou. The predominant genotypes of NoV during our study period are diverse. VP1 amino acid sequences of 2016-2017 GII.P16-GII.2 strains remain static after one year of circulation.
机译：2016年底，一个罕见的重组11个基因型称为GII.P16-GII.2导致亚洲和欧洲不同国家爆发的急剧增加，包括中国。然而，我们在2016年湖州冬季北摩洛鸠鲁病毒病例中没有观察到零星诺维病毒案件。因此，我们调查2016年1月至2017年浙江省湖州市2016年1月至2017年12月的散发性急性胃肠炎（年龄）案件的普遍和遗传多样性。从2016年1月到2017年12月，通过实时RT-PCR筛选了从年龄的患者中收集的1001次标本。通过RT-PCR扩增阳性样品的RNA依赖性RNA聚合酶（RDRP）和衣壳基因的部分序列并测序。通过在线Nov键入工具和系统发育分析证实了NOV的基因型。进一步获得本研究中检测到的GII.P16-GII.2的完整VP1序列并进行序列分析。共有204例（20.4％）标本被鉴定为Nov阳性。 GII GenoGroup占11月的大部分感染病例（98.0％，200/204）。 11个感染于所有年龄组（60岁）（60岁），5-15？年龄段的检测率最高（17/49,34.7％）。冬季春季可以看到11个感染的更高活性。主要的11个基因型已从GII.PE-GII.4 Sydney2012和GII.P17-GII.17转为GII.P16-GII.2，GII.PE-GII.4 SYDNEY2012和GII.P17-GII.17 2017年。系统发育分析显示，2016-2017 GII.P16-GII.2菌株与VP1区域中的日本2010-2012簇最密切相关，并且在HBGA结合位点的氨基酸中没有发现常见突变和预测表位。我们报告了GII.P16-GII.2菌株的出现，并在2017年1月至2017年12月期间的分子流行病学模式11载体感染。在我们的研究期间11月的主要基因型是多种多样的。 2016-2017 GII.P16-GII.2的VP1氨基酸序列在一年的循环后仍保持静止。

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