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首页> 外文期刊>BMC Infectious Diseases >Development of a convenient detection method for Trichomonas vaginalis based on loop-mediated isothermal amplification targeting adhesion protein 65
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Development of a convenient detection method for Trichomonas vaginalis based on loop-mediated isothermal amplification targeting adhesion protein 65

机译：基于环介导的等温扩增靶向粘合蛋白65的滴水瘤阴道的滴水菌的方便检测方法

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BACKGROUND:Trichomoniasis resulting from Trichomonas vaginalis (T. vaginalis) has been considered as a commonly seen disease with the transmission way of sex. At present, the detection methods of T. vaginalis mainly include wet mount microscopy, culture, PCR, immunofluorescence and ELISA. However, all of these detection methods exist shortcomings.METHODS:In this study, a loop-mediated isothermal amplification (LAMP) assay that targeted the species-specific sequence of adhesion protein 65 (AP65) gene had been conducted to detect T. vaginalis. The optimum reaction system and conditions were optimized in this rapid detection method.RESULTS:The results of sensitivity analysis showed that the LAMP assay targeting the AP65 gene was 1000 times more sensitive than the nested PCR targeting the actin gene commonly used for detection of T. vaginalis, and the detecting limitation of the former was 10 trichomonad. Moreover, the amplification of the target gene AP65 by LAMP assay exhibited high specificity and the product was exclusively from T. vaginalis. The detection technique of LAMP did not exhibit cross-reactivity with the common pathogens of Trichinella spiralis, Toxoplasma gondii, Escherichia coli, Candida albicans, Staphylococcus aureus, Haemophilus.CONCLUSIONS:According to the present study, the LAMP assay with the target of AP65 gene, was suitable for the early diagnosis of T. vaginalis infections. Consequently, the LAMP assay was proposed by the current study as a point-of-care examination and an alternative molecular tool which exhibited the potential value in the treatment, control and prevention of trichomoniasis transmission and relevant complication.
机译：背景：由richomonas阴道（T.AvinAnisis）产生的毛状霉病被认为是具有传递方式的常见疾病。目前，T.阴道的检测方法主要包括湿挂型显微镜，培养，PCR，免疫荧光和ELISA。然而，所有这些检测方法都存在缺点。在该研究中，已经进行了靶向粘合蛋白65（AP65）基因的物质序列的环介导的等温扩增（灯）测定以检测阴道。在这种快速检测方法中优化了最佳反应系统和条件。结果：敏感性分析结果表明，靶向AP65基因的灯测定比嵌套的PCR靶向靶向用于检测T.的肌动蛋白基因的1000倍。阴道，前者的检测限制是10个richomonad。此外，通过灯测定的靶基因AP65的扩增表现出高特异性，并且产品完全来自阴道。灯的检测技术没有表现出与Trichinella spirlalis，弓形虫的常见病原体的交叉反应性，弓形虫，大肠杆菌，念珠菌，金黄色葡萄球菌，血毛植物：根据本研究，灯测定AP65基因的靶标，适用于阴道阴道感染的早期诊断。因此，目前研究提出了目前的研究表明替代分子工具以及替代分子工具，其潜在价值在治疗，控制和预防滴虫病变和相关并发症。

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