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首页> 外文期刊>Botanical Studies >Arabidopsis and rice showed a distinct pattern in ZIPs genes expression profile in response to Cd stress
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Arabidopsis and rice showed a distinct pattern in ZIPs genes expression profile in response to Cd stress

机译：拟南芥和水稻在响应CD胁迫下显示拉链基因表达谱中的明显图案

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Abstract BackgroundPlant ZIP genes represent an important transporter family involved in metal transport. Evidence has implied that some ZIPs may contribute to plant Cd uptake, but a genome-wide examination of ZIPs’ role in Cd tolerance and uptake has rarely been reported. In this study, a genome-wide bioinformatic screening of candidate ZIP genes in Arabidopsis and rice was performed, followed by a systematic determination of their expression profile in response to Cd stress. Typical up-regulated ZIPs genes were then expressed in yeast cells to examine their effect on hosts’ Cd uptake.ResultsA total of 27 ZIP genes in Arabidopsis and rice were screened out based on sequence similarity. In Arabidopsis , Cd exposure strongly impacted the expression of most ZIPs, among which AtIRT1 , AtIRT2 , AtIRT4 AtZIP9 , AtZIP10 and AtZIP12 were sharply up-regulated and AtIRT3 , AtIRT5 were significantly down-regulated in root. In rice, all tested genes in shoot except for OsIRT1 and OsIRT12 were sharply up-regulated, while OsIRT1 and OsZIP1 in root were significantly down-regulated. Interestingly, some genes like AtIRT3 , AtZIP5 , AtZIP12 , OsIRT1 and OsZIP1 showed converse expression regulation when subject to the tested Cd stress. When expressed in yeast cells, three ZIPs, AtIRT1 , OsZIP1 and OsZIP3 , caused a substantial increase in Cd sensitivity and Cd accumulation of the host cells.ConclusionsIn conclusion, this study revealed a distinct pattern in ZIPs family genes expression between Arabidopsis and rice in response to Cd stress. Arabidopsis mainly up-regulated root ZIPs genes, while rice mainly up-regulated shoot ZIPs genes. Three genes, AtIRT1 , OsZIP1 and OsZIP3 , conferred an increased Cd accumulation and sensitivity to Cd stress when expressed in yeast cells, further implying their roles in Cd uptake in plants.
机译：摘要Backgroundplant Zip基因代表了参与金属运输的重要运输车家庭。有证据暗示一些拉链可能有助于植物CD摄取，但对耐湿性和摄取中的基因组在耐裂和摄取中的作用。在该研究中，进行了拟南芥和水稻中候选ZIP基因的基因组生物信息筛选，然后进行了系统测定其表达谱的响应于CD胁迫。然后在酵母细胞中表达典型的上调的拉链基因，以检查它们对宿主的CD吐痰的影响。基于序列相似性筛选拟南芥和水稻中的27个Zip基因的总和。在拟南芥中，CD暴露强烈影响大多数拉链的表达，其中ATIRT1，ATIRT2，ATIRT4 ATZIP9，ATZIP10和ATZIP12急剧上调，ATIRT3在根系中显着下调。在水稻中，除了OSIRT1和OSIRT12外的拍摄中的所有测试基因都被急剧上调，而OSIRT1和Root中的Oszip1显着下调。有趣的是，在ATIRT3，ATZIP5，ATZIP12，OSIRT1和OSZIP1等一些基因显示，当受测试的CD胁迫时显示逆表达调节。当在酵母细胞中表达时，三个ZIPS，ATIRT1，OSZIP1和OSZIP3导致宿主细胞的CD敏感性和CD积累的显着增加。结论，该研究揭示了拟南芥和水稻之间表达的拉链家族基因中的明显模式到CD压力。 Arabidopsis主要是上调根zips基因，而米饭主要上调射击ZIPS基因。三种基因，ATIRT1，oszip1和oszip3，在酵母细胞中表达时赋予CD累积和对Cd胁迫的敏感性，进一步意味着它们在植物中的CD摄取中的作用。

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