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首页> 外文期刊>BMC Musculoskeletal Disorders >Decreased angiogenic and increased apoptotic activities of bone microvascular endothelial cells in patients with glucocorticoid-induced osteonecrosis of the femoral head
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Decreased angiogenic and increased apoptotic activities of bone microvascular endothelial cells in patients with glucocorticoid-induced osteonecrosis of the femoral head

机译：血糖皮质激素诱导股骨头骨折患者患者骨微血管内皮细胞的血管生成和增加凋亡活性

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BACKGROUND:Glucocorticoid-induced osteonecrosis of the femoral head (ONFH) is closely associated with the dysfunction of the bone microvascular endothelial cells (BMECs). The present study investigated the angiogenic and apoptotic activity of the BMECs in glucocorticoid-induced ONFH.METHODS:This study enrolled a total of 12 patients, six of whom were assigned to the ONFH group whereas the other six served as the control group. The ONFH group was composed of patients with glucocorticoid-induced ONFH while the control group had femoral neck fractures. BMECs were isolated from the subchondral region of the femoral head. Cell proliferation, cell viability, tube formation assay, Transwell assay, TUNEL assay, and Western blot analysis were performed.RESULTS:BMECs of the two groups were successfully isolated and identified. No significant differences were noticed in BMECs proliferation between the two groups. However, compared to the control, cell viability, tube formation, and migration of BMECs were significantly decreased and the number of TUNEL positive cells was markedly increased in the ONFH group. In the ONFH group, it was also noted that the amount of Bax and cleaved-caspase3 was elevated while that of Bcl-2 was reduced.CONCLUSION:The findings of our study revealed that BMECs obtained from the glucocorticoid-induced ONFH patients had decreased angiogenic and increased apoptotic activities, which could explain the pathogenesis and progression of glucocorticoid-induced ONFH.
机译：背景技术：糖皮质激素诱导的股骨头（ONFH）的骨膜坏死与骨微血管内皮细胞（BMEC）的功能障碍密切相关。本研究研究了BMECs在糖皮质激素诱导的ONFH中的血管生成和凋亡活性。本研究共同注册了12名患者，其中六名患者被分配到ONFH集团，而另外六名曾经作为对照组。 ONFH组由糖皮质激素诱导的ONFH患者组成，而对照组具有股骨颈骨折。 BMEC与股骨头的子脚下区域分离。进行细胞增殖，细胞活力，管形成测定，Transwell测定，Turnel测定和Western印迹分析。结果：成功分离并鉴定了两组的BMEC。在两组之间的BMECS增殖中没有发现显着差异。然而，与对照，细胞活力，管形成和BMEC的迁移相比显着降低，在ONFH组中显着增加了TUNEL阳性细胞的数量。在ONFH组中，还注意到，BCL-2的抗叶片和切割的Caspase3的量升高。结论：我们的研究结果表明，从糖皮质激素诱导的ONFH患者中获得的BMEC减少了血管导生患者并增加凋亡活动，可以解释糖皮质激素诱导的ONFH的发病机制和进展。

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