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首页> 外文期刊>BMC Neuroscience >Differential effects of intragastric acid and capsaicin on gastric emptying and afferent input to the rat spinal cord and brainstem
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Differential effects of intragastric acid and capsaicin on gastric emptying and afferent input to the rat spinal cord and brainstem

机译：胃内酸和辣椒素对大鼠脊髓脑干胃排空和传入输入的差异效应

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Background Hydrochloric acid (HCl) is a potential threat to the integrity of the gastric mucosa and is known to contribute to upper abdominal pain. We have previously found that gastric mucosal challenge with excess HCl is signalled to the rat brainstem, but not spinal cord, as visualized by expression of c-fos messenger ribonucleic acid (mRNA), a surrogate marker of neuronal excitation. This study examined whether gastric mucosal exposure to capsaicin, a stimulant of nociceptive afferents that does not damage the gastric mucosa, is signalled to both brainstem and spinal cord and whether differences in the afferent signalling of gastric HCl and capsaicin challenge are related to different effects on gastric emptying. Results Rats were treated intragastrically with vehicle, HCl or capsaicin, activation of neurons in the brainstem and spinal cord was visualized by in situ hybridization autoradiography for c-fos mRNA, and gastric emptying deduced from the retention of intragastrically administered fluid. Relative to vehicle, HCl (0.5 M) and capsaicin (3.2 mM) increased c-fos transcription in the nucleus tractus solitarii by factors of 7.0 and 2.1, respectively. Capsaicin also caused a 5.2-fold rise of c-fos mRNA expression in lamina I of the caudal thoracic spinal cord, although the number of c-fos mRNA-positive cells in this lamina was very small. Thus, on average only 0.13 and 0.68 c-fos mRNA-positive cells were counted in 0.01 mm sections of the unilateral lamina I following intragastric administration of vehicle and capsaicin, respectively. In contrast, intragastric HCl failed to induce c-fos mRNA in the spinal cord. Measurement of gastric fluid retention revealed that HCl suppressed gastric emptying while capsaicin did not. Conclusion The findings of this study show that gastric mucosal exposure to HCl and capsaicin is differentially transmitted to the brainstem and spinal cord. Since only HCl blocks gastric emptying, it is hypothesized that the two stimuli are transduced by different afferent pathways. We infer that HCl is exclusively signalled by gastric vagal afferents whereas capsaicin is processed both by gastric vagal and intestinal spinal afferents.
机译：背景技术盐酸（HCl）对胃粘膜的完整性潜在威胁，并且已知有助于上腹部疼痛。我们以前发现用过量的HCl的胃粘膜攻击被用信号系统发出信号，而不是脊髓，通过C-Fos Messenger核糖核酸（mRNA）的表达，是神经元激发的替代标记。本研究检查了胃粘膜是否暴露于辣椒素，一种不会损害胃黏膜的伤害性传入的兴奋剂，信号都向发出脑干和脊髓，以及胃HCl和辣椒素攻击的累评信号是否与不同的影响有关胃排空。结果大鼠用载体，HCl或辣椒素处理，通过对C-FOS mRNA的原位杂交放射自显影来观察脑干和脊髓中神经元的激活，并从血管施用流体的保留中推断出胃排空。相对于载体，HCl（0.5μm）和辣椒素（3.2mm）分别通过7.0和2.1的因子增加了核泌瓜菌中的C-FOS转录。辣椒素也引起了尾胸脊髓的椎板I中的C-FOS mRNA表达的5.2倍上升，尽管该椎板中的C-FOS mRNA阳性细胞的数量非常小。因此，平均仅0.13和0.68个C-FOS mRNA阳性细胞分别在胃系和辣椒素的胃和辣椒素中的0.01mm部分中计数。相反，胃内HCL未能在脊髓中诱导C-FOS mRNA。胃液潴留的测量显示HCL抑制胃排空，而辣椒素没有。结论本研究的结果表明，HCl和辣椒素暴露于脑干和脊髓的胃粘膜暴露于脑干和脊髓。由于只有HCl阻断胃排空，因此假设两种刺激通过不同的传入途径转导。我们推断HCl由胃缩小传入排出HCl，而辣椒素通过胃迷肠和肠脊髓发育来处理。

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