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首页> 外文期刊>BMC Plant Biology >Transcriptomic study of pedicels from GA 3 -treated table grape genotypes with different susceptibility to berry drop reveals responses elicited in cell wall yield, primary growth and phenylpropanoids synthesis
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Transcriptomic study of pedicels from GA 3 -treated table grape genotypes with different susceptibility to berry drop reveals responses elicited in cell wall yield, primary growth and phenylpropanoids synthesis

机译：从Ga 3-治疗的表葡萄基因型与浆果下降的不同易感性的转录组，揭示了细胞壁产量，初级生长和苯丙醇合成中引发的反应

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Gibberellins (GA3) are the most sprayed growth regulator for table grape production worldwide, increasing berry size of seedless varieties through pericarp cell expansion. However, these treatments also exacerbate berry drop, which has a detrimental effect on the postharvest quality of commercialized clusters. Several studies have suggested that pedicel stiffening caused by GA3 would have a role in this disorder. Nevertheless, transcriptional and phenotypic information regarding pedicel responses to GA3 is minimal. Characterization of responses to GA3 treatments using the lines L23 and Thompson Seedless showed that the former was up to six times more susceptible to berry drop than the latter. GA3 also increased the diameter and dry matter percentage of the pedicel on both genotypes. Induction of lignin biosynthesis-related genes by GA3 has been reported, so the quantity of this polymer was measured. The acetyl bromide method detected a decreased concentration of lignin 7 days after GA3 treatment, due to a higher cell wall yield of the isolated fractions of GA3-treated pedicel samples which caused a dilution effect. Thus, an initial enrichment of primary cell wall components in response to GA3 was suggested, particularly in the L23 background. A transcriptomic profiling was performed to identify which genes were associated with these phenotypic changes. This analysis identified 1281 and 1787 genes differentially upregulated by GA3 in L23 and cv. Thompson Seedless, respectively. Concomitantly, 1202 and 1317 downregulated genes were detected in L23 and cv. Thompson Seedless (FDR??0.05). Gene ontology analysis of upregulated genes showed enrichment in pathways including phenylpropanoids, cell wall metabolism, xylem development, photosynthesis and the cell cycle at 7 days post GA3 application. Twelve genes were characterized by qPCR and striking differences were observed between genotypes, mainly in genes related to cell wall synthesis. High levels of berry drop are related to an early strong response of primary cell wall synthesis in the pedicel promoted by GA3 treatment. Genetic backgrounds can produce similar phenotypic responses to GA3, although there is considerable variation in the regulation of genes in terms of which are expressed, and the extent of transcript levels achieved within the same time frame.
机译：吉布林素（GA3）是全球最多喷涂的生长调节器，通过Pericarp细胞扩张增加无籽品种的浆果大小。然而，这些治疗也加剧了浆果下降，这对商业化群集的采后质量产生了不利影响。几项研究表明，GA3引起的PETICEL加强在该疾病中具有作用。然而，关于PECICEL对GA3的转录和表型信息是最小的。使用线路L23和汤普森无籽对GA3治疗的反应表征表明，前者比后者比后者更容易受到浆液的六倍。 Ga 3还增加了两种基因型上的Pepicel的直径和干物质百分比。已经报道了GA3的诱导木质素生物合成相关基因，因此测量该聚合物的量。由于Ga3处理的Pedicel样品的分离部分的较高细胞壁产率，乙酰溴化物在GA3处理后7天检测到降低的木质素浓度降低。因此，提出了响应于GA3的初始富集初级细胞壁组分，特别是在L23背景中。进行转录组分析以鉴定与这些表型变化有关的基因。该分析鉴定了1281和1787个基因在L23和CV中通过Ga 3差异上调。汤普森无籽，分别。同时，在L23和CV中检测到1202和1317下调基因。汤普森无籽（FDR？<？0.05）。上调基因的基因本体分析显示出在GA3施用后7天内致苯丙醇，细胞壁代谢，木瓜发育，光合作用和细胞周期的致致途径富集。通过QPCR表征12个基因，在基因型之间观察到突出差异，主要是在与细胞壁合成有关的基因中。高水平的浆果液滴与通过GA3处理促进的Peecec中的原发性细胞壁合成的早期强烈反应有关。遗传背景可以产生与GA3类似的表型反应，尽管在基因的调节方面具有相当大的变异，其表达的调节以及在同一时间框架内实现的转录水平的程度。

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