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首页> 外文期刊>Cancer Management and Research >LncRNA PCAT-1 Promoted ESCC Progression via Regulating ANXA10 Expression by Sponging miR-508-3p
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LncRNA PCAT-1 Promoted ESCC Progression via Regulating ANXA10 Expression by Sponging miR-508-3p

机译：LNCRNA PCAT-1通过通过冲压MIR-508-3P调节ANXA10表达来促进ESCC进展

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Background: Given the poor prognosis of metastatic esophageal squamous cell carcinoma (ESCC) patients, molecular mechanisms underlying the progression and metastasis of ESCC are highly desired in the scientific community. Prostate cancer associated transcript-1 (PCAT-1) is a lncRNA up-regulated in major types of cancers and is associated with the poor prognosis of cancer patients. This study aimed to understand the expression and role of PCAT-1 in the progression and metastasis of ESCC and to identify the potential lncRNA-miRNA interactions and signaling pathways underlying the mechanisms of action of PCAT-1 in ESCC. Materials and Methods: Gene expression levels were determined by qRT-PCR; protein levels were determined by Western blot assay; cell proliferation, invasion and migration were determined by CCK-8, Transwell invasion and wound healing assays, respectively; in vivo tumor growth was evaluated by xenograft nude mice model. Results: Our data showed the up-regulation of PCAT-1 in different human ESCC cell lines and in clinical ESCC tissues. Knockdown of PCAT-1 in ESCC cells significantly inhibited the proliferation, invasion and migration of the cancer cells. Moreover, we showed the interactions between PCAT-1 and miR-508-3p and demonstrated that PCAT-1 was able to repress miR-508-3p expression in ESCC cells via acting as a competing endogenous RNA. Besides, Annexin A10 (ANXA10) was identified to be the downstream target of the PCAT-1 and miR-508-3p interactions. Conclusion: This study demonstrated the functional role of PCAT-1 in promoting the proliferation, invasion and migration of ESCC cells. We also identified a PCAT-1/miR-508-3p/ANXA10 axis in mediating the promoting role of PCAT-1 in the progression of ESCC. The findings provide experimental evidence to support lncRNA PCAT-1 as a potential therapeutic target of ESCC.
机译：背景：鉴于转移性食管鳞状细胞癌（ESCC）患者的预后差，在科学界中，ESCC的进展和转移的潜在分子机制非常需要。前列腺癌相关的转录物-1（PCAT-1）是在主要类型癌症中上调的LNCRNA，与癌症患者的预后不良有关。本研究旨在了解PCAT-1在ESCC进展和转移中的表达和作用，并鉴定潜在的LNCRNA-miRNA相互作用和基于ESCC的作用机制的潜在的LNCRNA-miRNA相互作用和信号途径。材料和方法：通过QRT-PCR测定基因表达水平;蛋白质水平通过Western印迹测定法测定;通过CCK-8，Transwell侵袭和伤口愈合测定分别测定细胞增殖，侵袭和迁移;通过异种移植裸鼠模型评估体内肿瘤生长。结果：我们的数据显示不同人体ESCC细胞系和临床ESCC组织中PCAT-1的上调。在ESCC细胞中PCAT-1的敲低显着抑制了癌细胞的增殖，侵袭和迁移。此外，我们展示了PCAT-1和MIR-508-3P之间的相互作用，并证明PCAT-1能够通过作为竞争的内源RNA来压制ESCC细胞中的miR-508-3p表达。此外，鉴定了附睾A10（ANXA10）是PCAT-1和MIR-508-3P相互作用的下游靶标。结论：本研究证明了PCAT-1在促进ESCC细胞增殖，侵袭和迁移方面的功能作用。我们还识别了PCAT-1 / MIR-508-3P / ANXA10轴，调解PCAT-1在ESCC进展中的促进作用。结果提供了实验证据，以支持LNCRNA PCAT-1作为ESCC的潜在治疗靶标。

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