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首页> 外文期刊>Cancer Management and Research >Associations Between miRNAs and Two Different Cancers: Breast and Colon
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Associations Between miRNAs and Two Different Cancers: Breast and Colon

机译：miRNA和两种不同癌症之间的关联：乳房和结肠

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Objective: Screening approaches using microRNAs (miRNAs) have been gaining increased attention owing to their potential applications in the diagnosis, prognosis, and monitoring of cancer, because aberrant miRNA expression plays a role in the development and advancement of malignancies. The objectives of this study were to characterize mir21, miR31, mir143, mir145, and control RNU43, which are differentially expressed in peripheral blood mononuclear cells (PBMCs) of breast and colorectal cancer patients, compared to that in controls and to establish whether this is specific to breast and colon cancer for use as tumor markers. Methods: Thirty newly diagnosed patients with breast cancer and 30 patients with colorectal cancer were enrolled together with 30 healthy controls. PBMCs were isolated from venous blood samples of individuals. Next, miRNA expression analysis was performed by a two-step method of reverse transcription and qPCR. Results: The expression levels of miR-143 and miR-31 were significantly decreased, whereas the expression levels of miR-145 and miR-21 were significantly increased in breast cancer patients compared to those in healthy subjects. Moreover, the expression levels of miR-143, miR-145, and miR-21 were significantly increased and, in contrast, the changes in the expression levels of miR-31 were not statistically significant in colon cancer compared to those in healthy subjects. miR-21 exhibited the highest increase in both breast and colon cancers. There was a weak positive correlation between miR-145 and CA-15.3 in patients with breast cancer (r = 0.451; p = 0.012). miR-143 was positively correlated with the TNM stage in colon cancer patients (r = 0.568; p = 0.001). Conclusion: A biomarker panel composed of miR-21, miR-31, miR-143, and miR-145 in PBMC may provide a new diagnostic approach for the early detection of breast and colon cancer. As miR-21 expression was found to be the highest among all the miRNAs evaluated, it may represent a new tumor biomarker and a candidate therapeutic drug or gene target in colon and breast cancer.
机译：目的：筛选使用Micrornas（MiRNA）的方法由于患有癌症，预后和监测癌症的潜在应用而受到增加，因为异常miRNA表达在恶性肿瘤的发展和进步中发挥作用。该研究的目的是表征miR21，miR31，miR143，miR145和对照RNU43，其在乳腺癌和结肠直肠癌患者的外周血单核细胞（PBMC）中差异表达，与其在控制中相比，并建立了这是特异于乳腺癌和结肠癌用作肿瘤标志物。方法：30个新诊断患有乳腺癌患者和30名结肠直肠癌患者的患者与30例健康对照。从个体的静脉血液样本中分离出PBMC。接下来，通过逆转录和QPCR的两步方法进行miRNA表达分析。结果：miR-143和miR-31的表达水平显着降低，而miR-145和miR-21的表达水平与健康受试者相比，乳腺癌患者的表达水平显着增加。此外，MiR-143，miR-145和miR -21的表达水平显着增加，相比之下，与健康受试者的那些，MiR-31表达水平的表达水平的变化在结肠癌中没有统计学意义。 miR-21表现出乳腺癌和结肠癌的最高增加。 miR-145和Ca-15.3在乳腺癌患者（r = 0.451; p = 0.012）之间存在弱的正相关性。 miR-143与结肠癌患者的TNM阶段正相关（r = 0.568; p = 0.001）。结论：PBMC中的miR-21，miR-31，miR-143和miR-145组成的生物标志物面板可以为早期检测乳腺癌和结肠癌的新诊断方法提供新的诊断方法。由于发现miR-21表达是评估的所有miRNA中最高的，它可以代表新的肿瘤生物标志物和结肠癌和乳腺癌中的候选治疗药物或基因靶标。

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