...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cancer Management and Research >Long Noncoding RNA ZFPM2-AS1 Enhances the Malignancy of Cervical Cancer by Functioning as a Molecular Sponge of microRNA-511-3p and Consequently Increasing FGFR2 Expression
【24h】

Long Noncoding RNA ZFPM2-AS1 Enhances the Malignancy of Cervical Cancer by Functioning as a Molecular Sponge of microRNA-511-3p and Consequently Increasing FGFR2 Expression

机译：长度非编码RNA ZFPM2-AS1通过用作MicroRNA-511-3P的分子海绵来增强宫颈癌的恶性肿瘤，从而增加FGFR2表达

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Purpose: A long noncoding RNA called ZFPM2 antisense RNA 1 ( ZFPM2-AS1 ) has been verified as a key modulator in multiple human cancer types. Nonetheless, the expression and functions of ZFPM2-AS1 in cervical cancer remain poorly understood. Therefore, our purpose was to characterize the expression pattern, clinical value, and detailed roles of ZFPM2-AS1 in cervical cancer. Methods: Reverse-transcription quantitative PCR was carried out to measure ZFPM2-AS1 expression in cervical cancer. A Cell Counting Kit-8 assay, flow cytometry, Transwell migration and invasion assays, and a tumor xenograft experiment were conducted to determine the influence of ZFPM2-AS1 on cervical cancer cell proliferation, apoptosis, migration, and invasion in vitro and on tumor growth in vivo, respectively. Results: ZFPM2-AS1 was found to be aberrantly upregulated in cervical cancer, and its upregulation was associated with unfavorable values of clinical parameters. A ZFPM2-AS1 knockdown significantly reduced cervical cancer cell proliferation, migration, and invasion and increased apoptosis in vitro. The ZFPM2-AS1 knockdown decelerated tumor growth of cervical cancer cells in vivo. Molecular investigation indicated that ZFPM2-AS1 acts as a molecular sponge of microRNA-511-3p (miR-511-3p) in cervical cancer cells. Fibroblast growth factor receptor 2 ( FGFR2 ) mRNA was validated as a direct target of miR-511-3p in cervical cancer, and its expression was positively modulated by ZFPM2-AS1 . The effects of the ZFPM2-AS1 knockdown on malignant characteristics of cervical cancer cells were greatly attenuated by miR-511-3p inhibition. Conclusion: ZFPM2-AS1 promotes cervical cancer progression through upregulation of miR-511-3p–FGFR2 axis output, thereby pointing to possible diagnostics and therapeutics based on the ZFPM2-AS1 –miR-511-3p–FGFR2 pathway.
机译：目的：长期非编码RNA称为ZFPM2反义RNA 1（ZFPM2-AS1）已被验证为多种人类癌症类型的关键调节剂。尽管如此，宫颈癌中ZFPM2-AS1的表达和功能仍然明白。因此，我们的目的是在宫颈癌中表征ZFPM2-AS1的表达模式，临床价值和详细角色。方法：进行逆转录量化PCR以测量宫颈癌中的ZFPM2-AS1表达。进行细胞计数试剂盒测定，流式细胞术，转发迁移和侵袭测定，以及肿瘤异种移植物实验，以确定ZFPM2-AS1对体外宫颈癌细胞增殖，凋亡，迁移和侵袭的影响和肿瘤生长体内。结果：发现ZFPM2-AS1在宫颈癌中试样上调，其上调与临床参数的不利值有关。 ZFPM2-AS1敲低显着降低了宫颈癌细胞增殖，迁移和侵袭，血液凋亡增加。体内宫颈癌细胞的ZFPM2-AS1敲低肿瘤生长。分子研究表明，ZFPM2-AS1在宫颈癌细胞中作为MicroRNA-511-3P（miR-511-3P）的分子海绵。成纤维细胞生长因子受体2（FGFR2）mRNA被验证为宫颈癌中miR-511-3p的直接靶标，其表达通过ZFPM2-AS1正面调节。 ZFPM2-AS1敲低对宫颈癌细胞恶性特性的影响大大衰减了MIR-511-3P抑制作用。结论：ZFPM2-AS1通过U-511-3P-FGFR2轴输出的上调促进宫颈癌进展，从而指向基于ZFPM2-AS1-MIR-511-3P-FGFR2途径的可能的诊断和治疗方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号