...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Catalysts >Phylogeny and Structure of Fatty Acid Photodecarboxylases and Glucose-Methanol-Choline Oxidoreductases
【24h】

Phylogeny and Structure of Fatty Acid Photodecarboxylases and Glucose-Methanol-Choline Oxidoreductases

机译：脂肪酸光二羧酸盐和葡萄糖 - 甲醇 - 胆碱氧化酶的系统发育和结构

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Glucose-methanol-choline (GMC) oxidoreductases are a large and diverse family of flavin-binding enzymes found in all kingdoms of life. Recently, a new related family of proteins has been discovered in algae named fatty acid photodecarboxylases (FAPs). These enzymes use the energy of light to convert fatty acids to the corresponding C n-1 alkanes or alkenes, and hold great potential for biotechnological application. In this work, we aimed at uncovering the natural diversity of FAPs and their relations with other GMC oxidoreductases. We reviewed the available GMC structures, assembled a large dataset of GMC sequences, and found that one active site amino acid, a histidine, is extremely well conserved among the GMC proteins but not among FAPs, where it is replaced with alanine. Using this criterion, we found several new potential FAP genes, both in genomic and metagenomic databases, and showed that related bacterial, archaeal and fungal genes are unlikely to be FAPs. We also identified several uncharacterized clusters of GMC-like proteins as well as subfamilies of proteins that lack the conserved histidine but are not FAPs. Finally, the analysis of the collected dataset of potential photodecarboxylase sequences revealed the key active site residues that are strictly conserved, whereas other residues in the vicinity of the flavin adenine dinucleotide (FAD) cofactor and in the fatty acid-binding pocket are more variable. The identified variants may have different FAP activity and selectivity and consequently may prove useful for new biotechnological applications, thereby fostering the transition from a fossil carbon-based economy to a bio-economy by enabling the sustainable production of hydrocarbon fuels.
机译：葡萄糖 - 甲醇 - 胆碱（GMC）氧化还原酶是在所有王国中发现的大型和多样化的黄素结合酶。最近，已经在名为脂肪酸光二羧酸盐（FAPS）的藻类中发现了一种新的相关蛋白质。这些酶使用光的能量将脂肪酸转化为相应的C N-1烷烃或烯烃，并对生物技术应用具有巨大的潜力。在这项工作中，我们旨在揭示FAP的自然多样性及其与其他GMC氧化还原酶的关系。我们审查了可用的GMC结构，组装了GMC序列的大型数据集，发现一种活性位点氨基酸，组氨酸在GMC蛋白中非常良好地保守，但不含丙氨酸的FAP，其中不含普雷肽。使用本标准，我们发现了几种新的潜在FAP基因，包括基因组和偏见数据库，并显示出相关的细菌，古物和真菌基因不太可能是Faps。我们还确定了几种类似的GMC样蛋白质簇以及缺乏保守的组氨酸的蛋白质的亚颗粒，但不是faps。最后，对潜在光焦羧化酶序列的收集数据集的分析显示了严格保守的关键活性位点残留物，而Flavin腺嘌呤二核苷酸（FAD）辅因子和脂肪酸结合口袋附近的其他残留物更可变。所鉴定的变体可以具有不同的FAP活性和选择性，因此可以通过使烃燃料可持续生产来培养从化石碳的经济到生物经济的过渡到生物经济中有用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号