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Multiplexed DNA detection based on positional encoding/decoding with self-assembled DNA nanostructures

机译:基于单独的DNA检测与自组装DNA纳米结构的位置编码/解序

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Current multiplexed analysis methods suffer from either slow reaction kinetics (planar arrays) or complicated encoding/decoding procedures (suspension arrays). We report herein a multiplexed DNA detection strategy that addresses these issues, based on positional encoding/decoding with self-assembled DNA nanostructures. The strategy enables the acquisition of high-resolution, consistent, and quantitative assay results in a single round of a transmission electron microscopy imaging operation. Applications in polymerase chain reaction-free settings and assays of other structurally distinct targets can be anticipated through the implementation of the strategy with miniaturized femtoliter/attoliter dispensing technology and readily accessible DNA conjugate structures.
机译:电流多路复用分析方法遭受慢反应动力学(平面阵列)或复杂的编码/解码程序(悬浮阵列)。我们在本文中报告了一种基于用自组装DNA纳米结构的位置编码/解码解决这些问题的多路复用DNA检测策略。该策略使得能够获取高分辨率,一致性和定量测定导致一轮透射电子显微镜成像操作。通过实施具有小型化的Femtoliter / Attoliter分配技术的策略和易于访问的DNA共轭结构,可以预期在聚合酶链中的无反应性设置和其他结构明显靶标的测定。

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