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首页> 外文期刊>CHROMATOGRAPHY >Application of High-Speed Countercurrent Chromatography to the Separation of Flavanonol, Phenylcoumaran and Flavonolignans in Silybum marianum
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Application of High-Speed Countercurrent Chromatography to the Separation of Flavanonol, Phenylcoumaran and Flavonolignans in Silybum marianum

机译：高速逆流色谱法在黄烷醇，苯基苏仑和黄酮糖尿病中分离的应用

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摘要

Separation of flavanonol, phenylcoumaran and flavonolignans in Silybum marianum was examined using high-speed countercurrent chromatography (HSCCC). In order to prepare analytical standards, a flavanonol of aromadendrin (87.4% purity, 7.8 mg), three phenylcoumarans of jatrointelignan D (84.5% purity, 13.2 mg), dehydrodiconiferyl alcohol (76.1% purity, 1.4 mg) and dihydrodehydrodiconiferyl alcohol (93.0% purity, 5.8 mg), and three flavonolignans of silybin (88.8% purity, 35.6 mg), silydianin (99.3% purity, 25.1 mg) and silychristin (96.9% purity, 12.6 mg) were separated from the seeds of S. marianum using common column chromatography and ODS-HPLC, and identified by ~(1)H and ~(13)C NMR spectra. Then, HSCCC with the hexane/ethyl acetate/methanol/water (3 : 7 : 4 : 6, v/v) system was applied to the separation of aromadendrin, jatrointelignan D, silydianin and silybin. In this separation, it was revealed that silybin and silydianin were successfully separated from each other. The present HSCCC system was directly applied to the ethyl acetate extract and resulted in the separation of silybin. The overall results suggested that HSCCC is useful for the separation of bioactive compounds in S. marianum .
机译：使用高速逆流色谱（HSCCC）检查香脂醇，苯基苏马兰和黄酮核苷酸中的分离。为了制备分析标准，香料胺芳香素（纯度为87.4％，7.8mg），三种苯基氨基丙氨酸（纯度为84.5％，13.2mg），脱氢硅氧烷醇（76.1％纯度，1.4mg）和二氢氢二升醇（93.0％）纯度，5.8mg）和三种黄酮醇素（纯度为88.8％，35.6mg），含有Silydianin（99.3％纯度，25.1mg）和Silychristin（96.9％纯度，12.6mg）与 s的种子​​分离。 Marianum使用公共柱色谱和ODS-HPLC，并通过〜（1）H和〜（13）C NMR光谱鉴定。然后，用己烷/乙酸乙酯/甲醇/水（3：7：4：6，V / V）系统的HSCCC应用于芳香丹丁林，JatrointeLignan D，Silydianin和Silybin的分离。在这种分离中，揭示了甲岛和甲硅烷蛋白彼此成功分离。本发明的HSCCC系统直接施加到乙酸乙酯提取物上，导致甲硅素的分离。总体结果表明，HSCCC可用于在中分离生物活性化合物。 Marianum。

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