...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical and Translational Gastroenterology >Oncogenic Features in Histologically Normal Mucosa: Novel Insights Into Field Effect From a Mega-Analysis of Colorectal Transcriptomes
【24h】

Oncogenic Features in Histologically Normal Mucosa: Novel Insights Into Field Effect From a Mega-Analysis of Colorectal Transcriptomes

机译：组织普通粘膜中的致癌特征：从兆分析的兆分析域效应的新洞察

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

INTRODUCTION: Colorectal cancer is a common malignancy that can be cured when detected early, but recurrence among survivors is a persistent risk. A field effect of cancer in the colon has been reported and could have implications for surveillance, but studies to date have been limited. A joint analysis of pooled transcriptomic data from all available bulk RNA-sequencing data sets of healthy, histologically normal tumor-adjacent, and tumor tissues was performed to provide an unbiased assessment of field effect. METHODS: A novel bulk RNA-sequencing data set from biopsies of nondiseased colon from screening colonoscopy along with published data sets from the Genomic Data Commons and Sequence Read Archive were considered for inclusion. Analyses were limited to samples with a quantified read depth of at least 10 million reads. Transcript abundance was estimated with Salmon, and downstream analysis was performed in R. RESULTS: A total of 1,139 samples were analyzed in 3 cohorts. The primary cohort consisted of 834 independent samples from 8 independent data sets, including 462 healthy, 61 tumor-adjacent, and 311 tumor samples. Tumor-adjacent gene expression was found to represent an intermediate state between healthy and tumor expression. Among differentially expressed genes in tumor-adjacent samples, 1,143 were expressed in patterns similar to tumor samples, and these genes were enriched for cancer-associated pathways. DISCUSSION: Novel insights into the field effect in colorectal cancer were generated in this mega-analysis of the colorectal transcriptome. Oncogenic features that might help explain metachronous lesions in cancer survivors and could be used for surveillance and risk stratification were identified.
机译：简介：结直肠癌是一种常见的恶性肿瘤，可以在早期检测到时可以治愈，但幸存者之间的复发是一种持续的风险。据报道，癌症在癌症中的田间效应，并且可能对监测产生影响，但迄今为止的研究受到限制。进行来自所有可用的体积RNA测序数据组的合并转录组数据的联合分析，进行健康，组织学常规肿瘤邻近和肿瘤组织，以提供对场效不别的评估。方法：从筛选结肠透视从筛选结肠的活组织检查和来自来自基因组数据公杉的公开数据集和序列读取存档的新型批量RNA测序数据被认为是包含的。分析仅限于具有量化读取深度的样品至少为1000万读取。用鲑鱼估算转录物丰度，并在R的下游分析进行。结果：共有1,139个样品在3个队列中分析。主要队列由834个独立样本组成，来自8个独立数据集，包括462个健康，61个肿瘤相邻和311个肿瘤样品。发现肿瘤相邻的基因表达代表健康和肿瘤表达之间的中间状态。在肿瘤相邻样品中的差异表达基因中，1,143以类似于肿瘤样品的模式表达，并且这些基因富集用于癌症相关的途径。讨论：在本兆分析中，在结直肠转录组中产生了对结直肠癌田间效应的新洞察。致癌功能可能有助于解释癌症幸存者中的同叉病变，并可用于监测和风险分层。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号