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首页> 外文期刊>Acta Universitatis Sapientiae: Agriculture and Environment >First molecular identification of wheat seed gall nematode Anguina tritici races parasitized on wheat in Iraq
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First molecular identification of wheat seed gall nematode Anguina tritici races parasitized on wheat in Iraq

机译：第一次分子鉴定小麦种子胆线虫甲木制麦芽素族在伊拉克小麦上寄生化

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摘要

This study was conducted to identify races of wheat seed gall nematode Anguina tritici by using molecular identification for this nematode species parasitized on durum and bread wheat cultivars. Wheat seed galls of both cultivars were collected from two cities, Erbil and Duhok, from the Kurdistan Region and Iraq respectively. DNA was extracted from both nematode isolates (populations), and then PCR reactions were performed with Internal Transcribed Spacer (ITS) region using primers TW81/AB28 with 2 μl of template DNA of A. tritici for both nematode isolates on both wheat cultivars. The bands of both amplification products of PCR reactions were visualized in position about 800 bp on agarose gel, which indicates that both isolates of A. tritici belong to the same race. On the other hand, no nucleotide differences were observed between the two nematode isolations, as revealed by the sequence alignment of DNA of the internal transcribed spacer (ITS) region and phylogenetic tree, which emphasizes at the same time that both nematode isolates are of the same genetic structure or have the same identity and confirms their belonging to the same nematode race.
机译：通过使用在杜伦姆和面包小麦品种上寄生在杜兰姆和面包小麦品种上寄生的那种线虫物种来鉴定小麦种子胆线虫群体的比赛。从库尔德斯坦地区和伊拉克分别从两个城市，erbil和duhok收集两种品种的小麦种子血。从线虫分离株（群体）中提取DNA，然后使用引物TW81 / AB28与内部转录的间隔件（其）区域进行PCR反应，其中A颗粒中的2μL模板DNA。在两种小麦品种上，两种线虫分离株。 PCR反应的扩增产物的条带在琼脂糖凝胶上以约800bp的位置可视化，这表明A. tritici的分离株属于同一种族。另一方面，在两个线虫分离之间不观察到核苷酸差异，如内部转录的间隔（其）区域和系统发育树的DNA的序列比对所揭示的，这同时强调线虫分离物的同时相同的遗传结构或具有相同的身份，并确认它们属于同一线虫种族。

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