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首页> 外文期刊>ACS Omega >Supramolecular Host–Guest Chemistry-Based Folate/Riboflavin Functionalization and Cancer Cell Labeling of Nanoparticles
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Supramolecular Host–Guest Chemistry-Based Folate/Riboflavin Functionalization and Cancer Cell Labeling of Nanoparticles

机译：基于超分子的宿主 - 客体化学基础叶酸/核黄素官能化和纳米颗粒的癌细胞标记

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Nanoparticle-based cellular probes are commonly designed via covalent conjugation with affinity biomolecules. Those nanobioconjugates selectively interact with cell surface receptors and induce endocytosis followed by intracellular trafficking. However, this approach requires functional modification of biomolecules that may alter their biochemical activity. Here, we show that supramolecular host–guest chemistry can be utilized as an alternative approach in nanoparticle functionalization and selective cell labeling. We have used cyclodextrin-conjugated quantum dots (QDs) for supramolecular host–guest interaction-based functionalization with folate (QD-folate) and riboflavin (QD-riboflavin), where cyclodextrin acts as a host for the folate/riboflavin guest. We demonstrate that QD-folate and QD-riboflavin selectively label cells that have over-expressed folate/riboflavin receptors and induce the endocytosis pathway similar to covalently conjugated folate-/riboflavin-based nanoprobes. However, labeling is highly sensitive to the molar ratio of folate/riboflavin to cyclodextrin and incubation time. The presented functionalization/labeling approach is unique as it does not require covalent conjugation and may be extended for in vivo targeting application via simultaneous delivery of host and guest molecules.
机译：基于纳米粒子的细胞探针通常通过与亲和生物分子的共价缀合而设计。那些纳米核缀合物选择性地与细胞表面受体相互作用并诱导内吞作用，然后进行细胞内运输。然而，这种方法需要可以改变其生物化学活性的生物分子的功能性修饰。在这里，我们表明超分子宿主 - 客体化学可以用作纳米粒子官能化和选择性细胞标记中的替代方法。我们使用基于叶酸（QD-叶酸）和核黄素（QD-Riboflavin）的基于超分子宿主 - 访客相互作用的官能化的环糊精 - 缀合的量子点（QDS），其中环糊精作为叶酸/核黄素客人的宿主。我们证明了QD-叶酸和QD-核黄素选择性标记具有过表达的叶酸/核黄素受体的细胞，并诱导类似于共价缀合的叶酸/核黄素的纳米体的内吞作用途径。然而，标记对叶酸/ riboflavin至环糊精和孵育时间的摩尔比非常敏感。呈现的官能化/标记方法是独特的，因为它不需要共价缀合，并且可以通过同时递送宿主和客体分子在体内靶向应用中延伸。

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