...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>ACS Omega >Rapid and Sensitive Detection of Repetitive Nucleic Acid Sequences Using Magnetically Modulated Biosensors
【24h】

Rapid and Sensitive Detection of Repetitive Nucleic Acid Sequences Using Magnetically Modulated Biosensors

机译：使用磁性调制生物传感器的重复核酸序列的快速和灵敏度检测

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Repetitive DNA sequences are abundant in the genome of most biological species. These sequences are naturally “preamplified”, which makes them a preferential target for a variety of biological assays. Current methods to detect specific DNA sequences are based on the quantitative polymerase chain reaction (PCR), which relies on target amplification by Taq polymerase and uses a fluorescent resonance energy transfer (FRET)-based probe. Here, to rapidly detect a repetitive DNA sequence, we combine a highly sensitive magnetic modulation biosensing (MMB) system and a modified double-quenched FRET-based probe. The high numbers of copies of the female-specific XhoI sequence of the domestic chicken (Gallus gallus), combined with the low background fluorescence levels of the modified double-quenched probe and the high sensitivity of the MMB system, allow us to determine the chick sex in ovo within 13 min, with 100% sensitivity and specificity. Compared to quantitative PCR, the presented assay is 4–9 times faster. More broadly, by specifically tailoring the primers and probe, the proposed assay can detect any target DNA sequence, either repetitive or nonrepetitive.
机译：重复的DNA序列在大多数生物物种的基因组中丰富。这些序列是自然的“前置放大器”，其使它们成为各种生物测定的优先靶标。检测特异性DNA序列的目前的方法基于定量聚合酶链反应（PCR），其依赖于Taq聚合酶的靶扩增，并使用荧光共振能量转移（FRET）探针。这里，为了快速检测重复的DNA序列，我们将高敏感的磁调制生物传感（MMB）系统和改进的双骤冷的FRET探针组合。国产鸡肉（巨大Gallus）的女性特异性Xhoi序列的大量副本，结合改性的双淬火探头的低背景荧光水平和MMB系统的高灵敏度，让我们确定小鸡在13分钟内在ovo中进行性，灵敏度和特异性100％。与定量PCR相比，所呈现的测定速度快4-9倍。更广泛地，通过专门定制引物和探针，所提出的测定可以检测任何靶DNA序列，无论是重复的还是非批量。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号