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首页> 外文期刊>ACS Omega >Assessment of Molecular Mechanism of Gallate-Polyvinylpyrrolidone-Capped Hybrid Silver Nanoparticles against Carbapenem-Resistant Acinetobacter baumannii
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Assessment of Molecular Mechanism of Gallate-Polyvinylpyrrolidone-Capped Hybrid Silver Nanoparticles against Carbapenem-Resistant Acinetobacter baumannii

机译：基于Carbapemem抗性肺酸盐银纳米粒子杂交银纳米粒子的分子机制评估

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Acinetobacter baumannii is an opportunistic nosocomial pathogen and causes bacteremia, urinary tract infections, meningitis, and pneumonia. The emergence of drug-resistant strain makes most of the current antibiotics ineffective. It is high time to screen some therapeutics against drug-resistant strains. Plant-based medicines have recently emerged as one of the important therapeutic choices. Therefore, in the present study, we have screened the metabolites of Phyllanthus emblica, Ocimum tenuiflorum, and Murraya koenigii for their antibacterial effect against carabapenem-resistant strain (RS-307) of A. baumannii. The result showed that the methanolic extract of P. emblica inhibits the growth of RS-307. The composition of this extract was determined using phytochemical screening and nuclear magnetic resonance (1D and 2D-NMR). The mechanism of action of the plant extract was validated by estimating reactive oxygen species (ROS), lipid peroxidation, protein carbonylation, and membrane damage. The result showed that treatment with this extract showed a significant elevation in the production of ROS generations, lipid peroxidation, and protein carbonylation. This confirms that plant extract treatment confirmed ROS-dependent membrane damage mechanism. The NMR result showed the presence of ethyl gallate, ellagic acid, chebulagic acid, quercetin, flavonoid, and alkaloid. To validate the antimicrobial activity of the secondary metabolite (i.e., gallic acid), we synthesized gallate-polyvinylpyrrolidone-capped hybrid silver nanoparticles (G-PVP–AgNPs) and characterized using Fourier transform infrared spectroscopy (FTIR). G-PVP–AgNPs showed good antimicrobial activity against RS-307, and its mechanism of action was investigated using fluorescence and transmission electron microscopy and FTIR that confirmed ROS-dependent killing mechanism. Therefore, the present study highlighted and recommended the use of G-PVP–AgNPs as suitable therapeutics against carbapenem-resistant A. baumannii.
机译：Acinetobacter Baumannii是一种机会主义的医院病原体，导致菌血症，尿路感染，脑膜炎和肺炎。耐药菌株的出现使得大部分目前的抗生素无效。它是筛选一些治疗药物免受耐药菌株的时间。最近植物的药物作为重要的治疗选择之一。因此，在本研究中，我们筛选了Phyllanthus emblica，Octimum Tenuiflorum和Murraya Koenigii的代谢物，用于对A.Baumannii的抗菌菌株（RS-307）进行抗菌作用。结果表明，P. Emblica的甲醇提取物抑制了Rs-307的生长。使用植物化学筛选和核磁共振（1D和2D-NMR）测定该提取物的组成。通过估计反应性氧物质（ROS），脂质过氧化，蛋白质羰基化和膜损伤来验证植物提取物的作用机制。结果表明，用这种提取物处理在ROS世代，脂质过氧化和蛋白质羰基化的生产中显示出显着的升高。这证实了植物提取物治疗证实了ROS依赖性膜损伤机制。 NMR结果显示了吲哚，鞣果酸，煎蛋白，槲皮素，类黄酮和生物碱的存在。为了验证次级代谢物（即，Gallic acid）的抗微生物活性，我们合成了亲属 - 聚乙烯吡咯烷酮 - 封闭杂交银纳米粒子（G-PVP-AGNP），并用傅里叶变换红外光谱（FTIR）为特征。 G-PVP-AGNPS显示出对RS-307的良好抗微生物活性，并使用荧光和透射电子显微镜和FTIR来研究其作用机制，并确认依赖杀死杀伤机制。因此，本研究强调并建议使用G-PVP-AGNP作为针对Carbapenem抗性A. Baumannii的合适的治疗方法。

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