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首页> 外文期刊>African Journal of Biotechnology >Early detection of gray mold in grape using conventional and molecular methods
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Early detection of gray mold in grape using conventional and molecular methods

机译：使用常规和分子方法早期检测葡萄灰色模具

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Botrytis cinerea?affects grape quality and yield, and can be difficult to manage due in part to non-symptomatic, quiescent infection in berry development. The aim of this study was to develop a dual system for the detection, isolation and quantification of?B. cinerea. After three days of samples replication on the modified selective medium (mKERS), the results showed a significant infection effect on the majority of inflorescence samples, especially on the small berries which demonstrated?Botrytisinfection in all tested plants and appeared to be highly susceptible to?Botrytis?infection prior to harvest. Moreover, infection variation was determined in almost all inflorescence samples taken from different plants. The real-time PCR assay was used to determine the DNA quantity of?B. cinerea?in each sample tested. A linear relationship was found in these two systems, conventional and molecular assays, to demonstrate the infection of different samples with?B. cinerea. Although, the real-time PCR assay was highly expensive, it appeared to be more rapid and sensitive than the conventional selective medium assay, allowing both detection and quantification of?B. cinerea?within 3 h. However, conventional assay has an advantage of both detection and isolation of viable cells of?B.?cinerea, which resulted in making a wide collection of different isolates. Furthermore, this conventional assay is cheaper than molecular test, especially when we carry out a routine work. This dual method proved to be selective and sensitive assays and should be used to monitor?Botrytis?infection in the field.
机译：Botrytis cinerea？影响葡萄质量和产量，并且可能难以在浆果发育中的非对症性，静态感染的部分地位。本研究的目的是开发一种用于检测，隔离和量化的双系统？b。灰色。在修饰的选择培养基（MKERS）上的样品复制三天后，结果对大多数花序样品显示出显着的感染效应，特别是在显示的小浆果上？所有测试植物中的Botrytisinfection，并且似乎高度易受影响？ Botrytis？收获前的感染。此外，在几乎所有来自不同植物的花序样本中测定感染变异。实时PCR测定用于确定βb的DNA量。 Cinerea？在每个测试的样本中。在这两个系统中发现线性关系，常规和分子测定，以证明具有Δb的不同样品的感染。灰色。虽然，实时PCR测定非常昂贵，但它似乎比传统的选择性培养基测定更快速和敏感，允许检测和定量Δb。灰质？3小时内。然而，常规测定具有检测和分离的作用细胞的优点是？B.α的活细胞，导致制成各种不同的分离株。此外，这种常规测定比分子试验便宜，特别是当我们进行常规工作时。该双重方法证明是选择性和敏感的测定，应用于监测？Botrytis？在该领域的感染。

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