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首页> 外文期刊>African Journal of Biotechnology >Nitrite reductase (nii2) gene has a positive role in nitrogen metabolism in tobacco
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Nitrite reductase (nii2) gene has a positive role in nitrogen metabolism in tobacco

机译：亚硝酸盐还原酶（NII2）基因在烟草中的氮代谢中具有积极作用

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Nitrite reductase (NiR) is an enzyme that can reduce the concentration of nitrite. Nitrite is one of the precursors of tobacco-specific nitrosamines (TSNA), which are potentially carcinogenic products and exist only in tobacco and tobacco products. In this study, we analyzed the over expression?of ferredoxin-nitrite reductase?nii2 gene?(EC 1.7.7.1)?in tobacco to determine the reduction of nitrate and nitrite in response to its increase in the plants. Accordingly, the?Ntnii2?gene was cloned from tobacco based on the information from its wild-type (WT) homologous genes and it contained 1764 bp encoding the polypeptides of 587 amino acids. This gene was inserted into plant expression vector pBI121 and?Agrobacterium-mediated transformation results showed 23 transgenic tobacco lines through kanamycin resistance screening. After PCR-based screening,?Ntnii2?gene was confirmed to be successfully transcript and inherited 16 offspring’s and 8 lines were selected for further generation. The NiR mRNA levels,?in vivo?and?in vitro?NR activities were significantly higher in those selected eight transgenic lines than its counterpart WT and approximately 30% increase NiR activity in some transgenic lines. The nitrate content showed nearly 46% reductions. Although the nitrite contents were not considerably differed from that of non-transformed plants, most of the transgenic lines showed low nitrite level than wild-type plants. Therefore, transgenic plants with low level of nitrate and nitrite were produced.
机译：亚硝酸盐还原酶（NIR）是一种可以降低亚硝酸盐浓度的酶。亚硝酸盐是烟草特异性亚硝胺（TSNA）的前体之一，其潜在的致癌产品，仅存在于烟草和烟草制品中。在这项研究中，我们分析了过度表达？富勒森林 - 亚硝酸盐还原酶？NII2基因？（EC 1.7.1.1）？在烟草中，以反应其植物的增加，确定硝酸盐和亚硝酸盐的减少。因此，基于来自其野生型（WT）同源基因的信息，αNTNII2α基因基于烟草克隆，含有1764bp编码587氨基酸的多肽。将该基因插入植物表达载体PBI121中，并且介导的转化结果显示通过卡霉素抗性筛选23条转基因烟草线。基于PCR的筛选后，确认NTNII2？基因被证实成功转录并遗传16个后代和8根以进一步产生。 Nir mRNA水平，？在体内？在体外的α中，在选定的八个转基因系中显着高于其对应于WT，在一些转基因系中增加了约30％的NIR活性。硝酸盐含量显示近46％的降低。尽管亚硝酸盐含量与非转化植物的含量没有显着不同，但大多数转基因系显示出比野生型植物的低亚硝酸盐水平。因此，制备具有低水平硝酸盐和亚硝酸盐的转基因植物。

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