• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Characterization of water uptake and distribution in chickpea (Cicer arietinum L.) seeds during germination by NMR spectroscopy
【24h】

Characterization of water uptake and distribution in chickpea (Cicer arietinum L.) seeds during germination by NMR spectroscopy

机译：NMR光谱法萌发期间鹰嘴豆（Cicer Arietinum L.）种子的鸡豆摄取和分布的特征

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Experiments were conducted to characterize the changes in water status during imbibition by nuclear magnetic resonance (NMR) spectroscopy in chickpea seeds exposed to static magnetic fields of 100 mT for 1 h. Water uptake during seed germination showed three phases with rapid initial hydration phase I, followed by lag phase II and steady hydration phase III. Comparative analysis of the hydration pattern showed that water uptake was more in phase II and III in magnetically exposed than unexposed seeds. The longitudinal relaxation time (T1) of seed water showed significantly higher values and hence higher molecular mobility of cellular water in magnetically exposed seeds as compared to unexposed seeds. Analysis of transverse relaxation time (T2) revealed a three component of water in germinating chickpea seeds. Interesting observation found in this study was the early appearance of hydration water with least mobility and higher values of relaxation times of cytoplasmic bulk water and hydration water in magnetically treated over untreated seeds. Early hydration of macromolecules, membranes, greater molecular mobility of bulk and hydration water fractions in magnetically exposed seeds may be responsible for quicker germination and appearance of early seedling vigour in chickpea. Activities of enzymes related to germination process such as?a-amylase, dehydrogenase and protease were higher in magnetically exposed seeds as compared to unexposed seeds. Moreover, a significant correlation between the relaxation time of cytoplasmic bulk water and the activities of germination related enzymes supported our conclusion that this fraction of water plays a major role in the metabolism of germination process.
机译：进行实验，以表征在鸡膜种子中的核磁共振（NMR）光谱中吸收的水位变化，暴露于100mt的静态磁场1小时。种子萌发过程中的水分显示出三个阶段，快速初始水合相I，其次是滞后期II和稳定的水合作期III。水合模式的比较分析表明，在未曝光的种子上磁暴露的II期和III的水吸收更多。与未曝光的种子相比，种子水的纵向弛豫时间（T1）显示出显着更高的值，因此磁性曝光种子中的细胞水的较高分子迁移率。分析横向松弛时间（T2）揭示了发芽鹰嘴豆种子中的三种水。本研究中发现的有趣观察是水合水的早期外观，在未处理的种子上磁性处理的细胞质散装水和水合水的弛豫时间和弛豫时间较高。大分子，膜的早期水合，磁性曝光种子中的块状和水合水分的更大的分子迁移率可能是鹰嘴豆早期幼苗活力的更快萌发和出现的原因。与未曝光的种子相比，与萌发过程相关的酶如萌发过程，例如α-淀粉酶，脱氢酶和蛋白酶较高。此外，细胞质散装水的松弛时间与发芽相关酶的活性之间的显着相关性支持我们的结论，即该一部分水在发芽过程中的代谢中起着重要作用。

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号