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Redox-mediated polymerization and removal of benzidine from model wastewater catalyzed by immobilized peroxidase

机译:通过固定过氧化物酶催化催化废水中氧化还原介导的聚合和除去苯并氨酸

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Peroxidase from?Momordica charantia?was highly effective, active and stable for the oxidation of benzidine from model wastewater. There was no oxidative polymerization of benzidine without any redox mediator. Various experimental parameters were standardized for the maximum oxidation of benzidine by peroxidase. The maximum oxidation of this pollutant was observed in the presence of 0.05 mM phenol, 0.75 mM H2O2?and 0.2 U mL-1?bitter gourd peroxidase?(BGP)?in a buffer of pH 5.0 at 40°C. Comparative study was performed by soluble as well as surface immobilized bitter gourd peroxidase on Con A layered calcium alginate-starch beads for the degradation of benzidine from model wastewater.?Immobilized bitter gourd peroxidase was used for the successful and effective removal of water polluted with benzidine in batch as well as in continuous reactor. The effect of detergents and some water miscible organic solvent was also reported for the oxidation of benzidine from polluted water. Oxidation of benzidine in batch process by soluble and immobilized peroxidase was highly effective and it could remove 72 and 100% benzidine by soluble and immobilized bitter gourd peroxidase, respectively.?The reactor filled with immobilizedenzyme?retained more than 45% benzidine removal efficiency even after 30 days of its continuous operation.?The absorption spectra of the treated benzidine exhibited a marked difference in absorption at its?λmax?as compared to untreated benzidine polluted water.
机译:过氧化物酶来自苦瓜的苦曲肽?从模型废水中氧化苯胺的氧化是高效的,活性和稳定的。没有任何氧化还原介体的苯并胺没有氧化聚合。通过过氧化物酶的最大氧化酶标准化各种实验参数。在0.05mM酚,0.75mM H 2 O 2的存在下,观察到这种污染物的最大氧化α和0.2uml-1?粉末谷氧化酶?(BGP)?在40℃下pH 5.0的缓冲液中。通过可溶性以及表面固定化的表面固定的苦谷葡萄酸酶进行对比研究,用于从模型废水中降解苯并胺的分层藻酸钙 - 淀粉珠粒中。咪胶化苦瓜过氧化物酶用于成功,有效地去除用苯并甘地污染的水用批次以及连续反应器。还报道了洗涤剂和一些水混溶有机溶剂的效果用于从污染水中氧化苯胺。通过可溶性和固定化过氧化物酶氧化苯并甘氨酸的氧化是高效的,它可以分别通过可溶性和固定化的苦瓜过氧化物酶去除72和100%苯并氨酸。甚至均匀地填充有固定酶的反应器即使其连续操作的30天。治疗的苯并萘的吸收光谱在其λmax上的吸收差异显示出明显的差异?与未经处理的苯并甘油污染的水相比。

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