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首页> 外文期刊>African Journal of Biotechnology >Isolation and characterization of an Aux/IAA gene (LaIAA2) from Larix
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Isolation and characterization of an Aux/IAA gene (LaIAA2) from Larix

机译：落叶松的Aux / Iaa基因（Laiaa2）的分离和表征

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摘要

The phytohormone auxin controls many aspects of plant development. Auxin/indole-3-acetic acid (Aux/IAA) transcriptional factors are key regulators of auxin responses in plants. To investigate the effects of auxin on gene expression during the rooting process of?Larix?cuttings, a subtractive cDNA library was constructed and 272 UniEST were obtained by using suppression subtractive hybridization (SSH). Based on a fragment of 272 UniEST, the full-length cDNA of?LaIAA2, an?Aux /IAA?gene fromLarix?was isolated. Then, the response expression of?LaIAA2?to auxin was determined by treating with different sources and concentration of auxin and cycloheximide and the expression patterns of?LaIAA2?were examined in different tissues. The results show that?LaIAA2?appears to be the first response gene of auxin and?LaIAA2?gene was involved in the root development and auxin signaling. The express pattern of?LaIAA2?gene indicated that it?might play a central role in root development, specially regulated lateral and adventitious root production.
机译：植物激素植物素控制植物开发的许多方面。蟾蜍蛋白/吲哚-3-乙酸（AUX / IAA）转录因子是植物中养肝反应的关键调节因子。为了探讨植物素对生根过程的生成过程中的基因表达的影响吗？切割，通过使用抑制减法杂交（SSH）来构建减焦cDNA文库，272个大学。基于272个大学的片段，α1aiaa2的全长cDNA，aux / iaa？基因froblarix？是孤立的。然后，通过用不同来源和生长素和环己酰亚胺的不同来源和浓度和α1aa2的表达模式治疗αaaaa2的响应表达式。在不同的组织中检查了α1aiaa2的表达模式。结果表明？Laiaa2？似乎是蟾蜍蛋白的第一个反应基因和α1aia2？基因参与了根部发育和养肝信号传导。表达模式？laiaa2？基因表明它可能在根本发展中发挥着核心作用，特别规范的横向和不定根生产。

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