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Potential of Azotobacter vinelandii Khsr1 as bio-inoculant

机译：Azotobacter vinelancii khsr1作为生物植物的潜力

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The present study deals with the isolation and characterization of?Azotobacter vinelandii?Khsr1 from roots of the weed,?Chrysopogon aucheri, commonly known as golden beard grass indigenous to Khewra salt range, Pakistan and its evaluation as bio-inoculant. The population of the isolate varied from 107?to 108?cfu/g fresh weight of root. The preliminary identification of the isolate was made on the basis of carbon/nitrogen source utilization pattern as revealed by QTS-24 miniaturized identification system test which placed the isolate to the genus?Azotobacter. The 16S-rRNA partial sequence analysis confirmed the isolate as?A. vinelandii?strain?Khsr1. The 556 long nucleotide sequence of the isolate showed 98% similarity with?A. vinelandii?DJ (accession no. 012560.1). The isolate was capable of producing phytohormones: indole-3-acetic acid, gibberellic acid, trans-zeatin riboside and abscisic acid in culture supernatant, stimulated growth of?Zea mays?L. seedlings and augmented proline content of roots and shoots both under normal and NaCl stressed conditions. However, the magnitude of stimulation is higher under un-stressed condition.
机译：本研究涉及杂草根源的孤独和表征？来自杂草的根，？Chrysopogon Aucheri，通常被称为金胡子草本土，对Khewra Salt Range，巴基斯坦及其作为生物植物的评价。分离物的群体从107变化？到108？CFU / g的根的重量。在QTS-24小型化鉴定系统测试所揭示的碳/氮源利用模式的基础上，对分离物的初步鉴定进行了QTS-24小型化鉴定系统试验，其将分离物与属αα斑曲杆菌。 16S-RRNA部分序列分析证实了分离物为a。 vinelandii？菌株？khsr1。分离物的556个长的核苷酸序列显示出98％的相似性与Δa。 vinelandii？DJ（加入编号012560.1）。分离物能够生产植物激素：吲哚-3-乙酸，甘油酸，反式颗粒核糖苷和培养上清液中的脱落酸，刺激的β1。幼苗和增强脯氨酸含量的根部，并在正常和NaCl胁迫条件下射击。然而，在不强调的情况下刺激的幅度较高。

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